水飞蓟素对人胃癌SGC 7901细胞增殖、迁移和侵袭的抑制作用及其机制

目的：探讨水飞蓟素对人胃癌SGC 7901细胞增殖、迁移及侵袭的影响和促凋亡作用，阐明其可能机制。方法：选取对数生长期的人胃癌SGC 7901细胞，分为对照组和不同浓度水蓟素组（分别给予15、30和60 mg·L^-1水飞蓟素），倒置显微镜观察各组细胞形态表现，MTT法检测细胞周期和细胞凋亡率，流式细胞术检测细胞增殖抑制率，划痕实验和Transwell小室检测细胞迁移和侵袭能力。结果：水飞蓟素作用SGC 7901细胞24 h后，与对照组比较，30和60mg·L^-1水飞蓟素组细胞贴壁密度变小，细胞形态不规则、体积变小，产生大量细胞碎片。与对照组比较，不同浓度水飞蓟素组细胞增殖抑制率明显升高（P<0.05），G₁期细胞百分率和细胞凋亡率明显升高（P<0.05），细胞迁移距离明显缩短（P<0.05），穿膜细胞数量明显降低（P<0.05）。结论：水飞蓟素对人胃癌SGC 7901细胞的增殖抑制作用可能是通过诱导G₁期细胞阻滞和早期凋亡引起的。

Inhibitory effects of silymarin on proliferation,migration and invasion of human gastric cancer SGC 7901 cells and their mechanisms

Objective:To investigate the effects of silymarin on the proliferation, migration and invasion of human gastric cancer cell line SGC 7901 and its role in promoting apoptosis,and to clarify their possible mechanisms. Methods:The human gastric cancer SGC 7901 cells in logarithmic growth phase were selected and divided into control group (without silymarin) and different concentrations(15,30, and 60 mg·L^-1)of silymarin groups.Inverted microscope was used to observe the morphology of cells.MTT method wasused to detect the cell cycle and the apoptotic rates of cells in various groups.The inhibitory rates of proliferation of cells in various groups were detected by flow cytametry. Scratch test and Transwell chamber assay were used to detect the cell migration and invasion abilities. Results:After the SGC 7901 cells were treated with silymarin for 24 h, compared with control group, the adherent densities of the cells in 30 and 60 mg·L^-1 silymarin groups were smaller,the cell shape was irregular and the volume was smaller, resulting in a large number of cell debris.Compared with control group, the inhibitory rates of cells in different concentrations of silymarin groups were increased significantly(P<0.05); the percentages of cells in G₁ phase and the apoptosis rates of SGC 7901 cells were increased significantly(P<0.05);the migration distances were decreased(P<0.05) and the number of transmembrane cells was decreased significantly(P<0.05). Conclusion:Silymarin can inhibit the proliferation of human gastric cancer SGC 7901 cells by inducing the G₁ phase cell arrest and early apoptosis.