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兔骨髓间充质干细胞向成骨方向的诱导分化及体内应用
引用本文:韩冬,李建军,张巨,孙鸿斌,王健,刘建国,王国君.兔骨髓间充质干细胞向成骨方向的诱导分化及体内应用[J].吉林大学学报(医学版),2004,30(1):79-81.
作者姓名:韩冬  李建军  张巨  孙鸿斌  王健  刘建国  王国君
作者单位:1. 吉林大学中日联谊医院手外科,吉林 长春130031;2.吉林大学第一医院骨科,吉林 长春130021;3. 温州医学院附属第一医院骨科,浙江 温州 325000
摘    要:目的:研究经矿化诱导的兔骨髓间充质干细胞(MSC)在裸鼠体内的成骨潜能。方法:分离并培养兔MSC,矿化诱导,观察MSC的形态学改变及碱性磷酸酶(ALP)活性。将诱导后的MSC-去抗原牛松质骨复合体植入裸鼠背部皮下,3 d后取材,行组织学观察。结果:经矿化诱导后,MSC的形态由长梭形向多角形和三角形转化,ALP组织化学染色阳性,活性明显增高。MSC-去抗原牛松质骨复合体植入裸鼠后3 d,组织学见细胞均匀贴附于骨小梁表面,伸展良好,未见未贴壁细胞及外源性纤维组织、血管长入。结论:经矿化诱导的MSC向成骨细胞方向分化和增殖,经观察MSC-去抗原牛松质骨复合体在裸鼠体内可成活并增殖。

关 键 词:间充质干细胞  成骨细胞  去抗原异种松质骨  疾病模型  动物    
文章编号:1671-587X(2004)01-0079-03
收稿时间:2003-05-21
修稿时间:2003年5月21日

Experimental study of osteoblastic differentiation of rabbit messenchymal stem cell in vitro and in vivo
HAN Dong,LI Jian-jun,ZHANG Ju,SUN Hong-bin,WANG Jian,LIU Jian-guo,WANG Guo-jun.Experimental study of osteoblastic differentiation of rabbit messenchymal stem cell in vitro and in vivo[J].Journal of Jilin University: Med Ed,2004,30(1):79-81.
Authors:HAN Dong  LI Jian-jun  ZHANG Ju  SUN Hong-bin  WANG Jian  LIU Jian-guo  WANG Guo-jun
Institution:1. Deptartment of Hand Surgery, China-Japan Union Hospital, Jilin University, Changchun 130031, China;2. Department of Orthopaedics, First Hospital, Jilin University, Changchun 130031, China;3. Department of Orthopaedics, First Hospital, Wenzhou Medical College,Wenzhou 325000, China
Abstract:Objective\ To study the osteogenic property of the induced rabbit bone messenchymal stem cells(MSC) in nude rats.Methods MSC were obtained by using gradient centrifuge method. The cells had been cultured in mineralized medium for 1 week, then the ALP activity was observed. The induced MSC/antigen extracted bovine cancellous bone were implanted in the nude mice subcutaneously. At 3rd day after implantation, the implants underwent the histological analysis. Results ALP activity was higher and the cells displayed fibroblast like morphology, revealed positive staining with alkaline phosphatase method in the induced medium. At 3rd day after implantation, histological analysis revealed that the induced cells were adhered at the surface of the bone trabeculase and growed well. Exogenous fibro tissue, blood vessel and free induced cells were invisible. Conclusion Rabbit MSC could differentiate into osteoblast and proliferate induced by the mineral medium. The induced MSC/antigen extracted bovine cancellous bone could survive in the nude rat.
Keywords:bone marrow cells  messenchymal stem cell  osteoblasts  antigen  extracted xenogeneic cancellous bone  disease models  animal
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