20(s)-原人参二醇对前列腺癌RM-1细胞的生长抑制作用及其机制

目的：观察20(s)-原人参二醇（PPD）对前列腺癌RM-1细胞生长的影响，并探讨其作用机制。方法：建立前列腺癌RM-1细胞动物模型，将36只C57小鼠随机分为3组：模型组（橄榄油等体积灌胃每天1次、生理盐水等体积腹腔注射每周1次）,紫杉醇（Taxol）组（20 mg/kg，腹腔注射每周1次），PPD组（20 mg/kg，灌胃每天1次），连续给药4周。每隔3 d测量肿瘤体积1次，给药第4周末处死动物。观察指标：动物一般状态、肿瘤体积、瘤重、抑瘤率、死亡率，采用RT-PCR和Western blotting法检测肿瘤组织Cyclin D1蛋白表达水平。结果：PPD组小鼠一般状态明显好于模型组，表现为活泼好动、毛色光亮；而模型组小鼠步履蹒跚、行动迟缓、精神萎靡；肿瘤组织出现破溃、糜烂、出血。给药13 d时，PPD组小鼠肿瘤体积明显减小（P<0.05）；21 d时PPD组肿瘤体积明显小于Taxol组(P<0.05）。与模型组比较，PPD组小鼠瘤质量明显降低（P<0.05），死亡率为零；与Taxol组比较，PPD组小鼠抑瘤率增高（P<0.05），死亡率降低（P<0.05）。各给药组小鼠肿瘤组织Cyclin D1基因转录、蛋白表达水平均明显低于模型组（P<0.05）,与Taxol组比较，PPD组Cyclin D1基因转录明显减弱（P<0.05），而蛋白表达水平差异无显著性（P>0.05）。结论：PPD有明显抑制前列腺癌RM-1细胞生长的作用，其作用机制可能与下调Cyclin D1蛋白表达有关。

Growth inhibition of 20 (s) -protopanaxadiol on prostate cancer cells RM-1 in mice and its mechanism

Objective To observe the effects of 20(s)-protopanaxadiol (PPD) on the growth of prostate cancer cells RM-1 and explore the mechanism of its action.Methods The mouse models with prostate cancer were constructed with RM-1 cells.36 mice C57 were randomly divided into three groups:model group (fed with an equal volume of olive oil once a day,an equal volume of normal saline injection intraperitoneal once a week),paclitaxel (Taxol) group (20 mg·kg~(-1),intraperitoneal injection once a week),PPD group (20 mg·kg~(-1),ig once a day),continuous administration of 4 weeks.The tumor size was measured once every 3 d,and the morphological changes of tumor were observed,the animals were killed over the fourth weekend.Observation:the general state of animal tumor weight,tomor volume,inhibitory rate,mortality;and the expression of Cyclin D1 gene was detected with RT-PCR and Western blotting.Results Compared with model group,the tumor growth of mice in other groups significantly slowed down,and the mice in PPD group had bright hair.The mice in model group scrambled and moved slowly with tumor ulceration,erosion and hemorrhage.The tumor volume of the mice in PPD group was significantly smaller than that in model group (P<0.05) 13 d after administration,and it was also significantly smaller than that in Taxol group (P<0.05) 21 d after administration.Compared with model group,the tumor weight of the mice in PPD group was significantly decreased (P<0.05),but the inhibitory rate was significantly increased (P<0.05),no death.Compared with Taxol group.the inhibitory rate in PPD group was significantly increased (P<0.05),the mortality was significantly decreased (P<0.05).The RT-PCR and Western blotting results showed that Cyclin D1 gene and protein expressions in treatment groups were significantly reduced (P<0.05) compared with model group compared with Taxol group,the expression of Cyclin D1 in PPD group was significantly decreased (P<0.05),but there was no significant difference of Cyclin D1 protein expression between PPD group and Taxol group (P>0.05).Conclusion PPD can significantly inhibit the growth of prostate cancer cells RM-1 and its mechanism may be related to the down-regulation of the expression of Cyclin D1.