中草药远志对环磷酰胺所致小鼠遗传物质损伤的保护作用和淋巴细胞功能的增强作用

目的:探讨中草药远志抗突变作用和对脾T淋巴细胞增殖的影响。方法:小鼠骨髓嗜多染红细胞微核实验：30只小鼠随机分为6组（每组5只），即阴性对照组( NS)、环磷酰胺(CP)组(30 mg·kg^-1)及远志抗诱变组(远志0.5、1.0、2.0、4.0 g·kg^-1+CP 30 mg·kg^-1)，按改良方法制片检测微核频率；淋巴细胞转化实验：24只小鼠随机分为4组（每组6只），即生理盐水组、CP组(30 mg·kg^-1)、远志水煎液组（2.0 g·kg^-1）及远志水煎液+CP组（2.0 g·kg-1远志+CP 30 mg·kg^-1），采用MTT法计算刺激指数(SI)。结果:阴性对照组、远志各剂量组的微核率低于CP组(P<0.05)；生理盐水组、远志水煎液组、远志水煎液+CP组、CP组的SI分别为2.11±0.13、2.37±0.18、1.97±0.06和1.34±0.19；远志水煎液组的SI生理盐水组的SI比较差异有显著性（P<0.05）；远志水煎液+CP组SI与CP组比较差异有显著性（P<0.05）。结论:远志具有抗诱变作用，即对遗传物质具有保护作用；远志能提高昆明种小鼠脾细胞对ConA的反应性,并能改善由CP所致的小鼠脾细胞ConA反应性降低，即远志能提高淋巴细胞功能。

Protective effect of Polygala tenuifolia willd on genetic damage and enhancement of lymphocyte function induced by cyclophosphamide in mice

Objective To study the effect of anti-mutation of Chinese medicinal herb Poly gala tenui folia willd on T lymphocyte proliferation in spleen. Methods The micronucleus test of mouse bone marrow cell (MNT): thirty mice were divided into six groups (n = 5) , negative control (NS) , cyclophosphamide group (CP 3. 0 mg · kg-1) , Poly gala tenui folia willd antimutagenesis groups (Polygala tenui folia willd with dosage of 0.5, 1.0. 2.0, 4.0 g · kg-1 + CP 30 mg · kg-1 ). The improved method was used to detect the micro-nuclei frequency. Lymphocyte transformation test: twenty-four mice were divided into four groups (n = 6), saline control , CP control (3.0 mg · kg-1), Polygala tenui folia willd (2.0 g · kg-1), Polygala tenui folia willd + CP (2. 0 g · kg-1 Polygala tenuifolia willd + CP 30 mg · kg- ) group. MTT assay was used to calculate the stimulation index (SI). Results The micro-nuclei frequency was significant difference between Poly gala tenui folia willd antimutagenesis groups and CP groups (P<0. 05); the SI in saline control, Polygala tenuifolia willd, Polygala tenuifolia willd + CP, CP groups were 2. 11 ± 0. 13, 2. 37± 0. 18, 1. 97 ± 0. 06 and 1. 34 ± 0. 19, respectively, the difference of SI was significant between Polygala tenuifolia willd and saline control groups (P< 0. 05). The difference of SI was significant between Polygala tenuifolia willd + CP and CP groups (P<0. 05). Conclusion Polygala tenuifolia willd may protect genetic material from injury caused by CP, namely, showing effective antimutagenesis and yet Polygala tenuifolia. willd may increase the reactivity of mouse spleen cells to ConA and could improve reactivity reduction induced by CP, namely Polygala tenuifolia willd could enhance lymphocyte function.