山楂叶总黄酮对实验性心肌梗死犬的保护作用

目的: 探讨山楂叶总黄酮对实验性心肌梗死犬的保护作用。 方法: 36只健康杂种犬,随机分为假手术组、模型组(给予相同体积的生理盐水)、复方丹参注射液组(1.65 g·kg^-1)和山楂叶总黄酮低、中、高剂量组(5、10和20 mg·kg^-1),每组6只,静脉滴注给药。采用麻醉后结扎犬冠状动脉前降支方法建立急性心肌梗死模型。于给药后30、60、90、120、180、240、300和360 min记录各组犬心外膜电图,检测血清中天冬氨酸氨基转移酶(AST)、肌酸磷酸激酶(CPK)、乳酸脱氢酶(LDH)和超氧化物歧化酶(SOD)活性以及丙二醛(MDA)水平,计算ST段升高总和(∑ST)及心肌梗死范围(MIS)。 结果: 与假手术组比较,模型组犬血清中AST、CPK、LDH和MDA水平升高(P<0.05),SOD活性降低(P<0.05),全心重百分比、心室重百分比及∑ST升高(P<0.05或P<0.01);与模型组比较,山楂叶总黄酮中、高剂量组犬血清中AST、CPK、LDH和MDA水平降低(P<0.05或P<0.01),SOD活性升高(P<0.05或P<0.01),∑ST降低(P<0.05或P<0.01),山楂叶总黄酮低、中、高剂量组犬的全心重百分比和心室重百分比均降低(P<0.05或P<0.01)。 结论: 山楂叶总黄酮对实验性心肌梗死犬具有保护作用。

Protective effect of Hawthorn Leaves Flavonoids on experimental myocardial infarction in dogs

Objective: To study the protective effect of Hawthorn Leaves Flavonoids (HLF)on the experimental myocardial infarction in the dogs,and to clarify its mechanism.Methods: Thirty-six healthy dogs were randomly divided into sham operation group,model group (given the same volume of 0.9% saline),Compound Danshen Injection group (1.65 g·kg^-1),and low,medium and high doses of HLF groups (5,10,20 mg·kg^-1),(n=6);intravenous injection was performed. The method of ligating the anterior descending coronary artery was used to establish acute myocardial infraction models of dogs after anesthesia. The epicardial electrical diagram at 30,60,90,120,180,240,300,360 min of the dogs in various groups afer administration were recorded. The activities of serum aspartate aminotransferase (AST),creatine phosphokinase (CPK),lactate dehydrogenase (LDH),superoxide dismutase (SOD)and malondialdehyde (MDA)levels of the dogs in various groups were detected,and the extent of myocardial ischemia (ΣST)and myocardial infarct size (MIS)were calculated. Results: Compared with sham operation group,the serum AST,CPK,LDH activities and MDA level of the dogs in model group were increased (P<0.05),the SOD activity was decreased (P<0.05),and the whole heart weight percentage,ventricular weight percentage and ∑ST were increased (P<0.05 or P<0.01).Compared with model group,the serum AST,CPK,LDH activities and MDA levels of the dogs in medium and high doses of HLF groups were decreased (P<0.05 or P<0.01),the SOD activities were increased (P<0.05 or P<0.01),and the ΣST were decreased (P<0.05 or P<0.01);the whole heart and ventricular weight percentages of the dogs in low,medium,and high doses of HLF groups were decreased (P<0.05 or P<0.01). Conclusion: Hawthorn Leaves Flavonoids has the protective effect on experimental myocardial infarction in the dogs.