五味子乙素对ICR小鼠的抗焦虑作用及其作用机制

目的：探讨五味子乙素（SchB）对ICR小鼠的抗焦虑作用，并阐明其相关机制。方法：ICR小鼠分为空白对照组（灌胃给予双蒸水）、2.5 mg·kg^-1SchB组、5.0 mg·kg^-1SchB组和10.0 mg·kg^-1SchB组（灌胃给予相应剂量SchB）。各组小鼠连续灌胃给药7d后，进行十字迷宫及零迷宫实验；实验结束后，取空白对照组及SchB 10 mg·kg^-1组小鼠外周血及脑组织。采用酶联免疫吸附试验检测小鼠外周血和脑组织中γ氨基丁酸（GABA）及谷氨酸（Glu）水平，计算GABA/Glu比值；采用Western blotting法检测小鼠脑组织中GABAA受体α1亚单位（GABAARα1）与GABAA受体γ2亚单位（GABAARγ2）的表达水平。结果：与空白对照组比较，5.0和10.0 mg·kg^-1SchB组小鼠在高架十字迷宫中进入开臂的次数百分率明显增加（P<0.05或P<0.01），开臂滞留时间百分率明显增加（P<0.05或P<0.01），在高架零迷宫中进入开臂次数百分率明显增加（P<0.01），开臂探头次数百分率明显增加（P<0.01）。与空白对照组比较，10.0 mg·kg^-1SchB组小鼠外周血及脑组织中GABA水平明显增加（P<0.01），Glu水平明显降低（P<0.01），GABA/Glu的比值明显升高（P<0.01），脑组织中GABAA Rα1和GABAA Rγ2表达水平明显升高（P<0.01）。结论：SchB对小鼠具有抗焦虑作用，该作用可能与调节外周血和脑组织中GABA和Glu水平及脑组织中GABAARα1和GABAARγ2表达水平有关。

Antianxiety effect of schisandrin B in ICR mice and its mechanism

Objective:To investigate the antianxiety effect of schisandrin B (SchB) in the ICR mice,and to elucidate its related mechanisms.Methods:The ICR mice were divided into blank control group (administered with distilled water intragastrically),2.5 mg · kg-1 SchB group,5 mg · kg-1 SchB group,and 10 · mg · kg-1 SchB group (administered with SchB intragastrically).After the successive administration for 7 d,cross maze test and zero maze test were conducted in all the mice,and then the peripheral blood and brain tissue samples of the mice in blank control group and 10 mg · kg-1 SchB group were taken.The levels of γ-aminobutyric acid (GABA) and glutamic acid (Glu) in the peripheral blood and brain tissue of the mice were detected by ELISA,and the ratio of GABA/Glu was calculated.The expression levels of the α1 subunit of the γ-aminobutyric acid (GABAARα1) and the γ2 subunit of the γ-aminobutyric acid (GABAARγ2) in the brain tissue of the mice were detected by Western blotting method.Results:In the elevated plus maze test,the percentages of number of open arm entries in 5.0 and 10.0 mg · kg 1 SchB groups were significantly increased compared with blank control group (P＜0.05 or P=0.01),and the percentages of time of open arm entries were significantly increased (P=0.05 or P＜0.01).In the elevated zero maze test,the percentages of number of open arm entries of the mice in 5.0 and 10.0 mg · kg-1SchB groups were significantly increased compared with blank control group (P＜0.01),and the percentages of number of entering open arm probe were significantly increased (P＜0.01).Compared with blank control group,the GABA levels in the peripheral blood and brain tissue of the mice in 10.0 mg · kg-1 SchB group,were significantly increased (P＜0.01),the Glu levels in the peripheral blood and brain tissue were significantly reduced (P＜0.01),the ratios of GABA/Glu were significantly increased (P＜0.01),and the expression levels of GABAARα1and GABAARγ2 in the brain tissue were significantly increased (P＜0.01).Conclusion:SchB has an antianxiety effect in the mice,and the effect may be related to its regulating the levels of GABA and Glu in the peripheral blood and brain tissue and the expression levels of GABAA Rα1 and GABAA Rγ2 in the brain tissue.