肉苁蓉醇提物对去卵巢骨质疏松大鼠的治疗作用及其机制

目的：探讨肉苁蓉醇提物（CDE）对骨质疏松大鼠的治疗作用，阐明其作用机制。方法：72只雌性SD大鼠随机分为对照组，模型组，雌二醇组（0.3 mg·kg^-1），低、中和高剂量（0.5、1.0和2.0 g·kg^-1） CDE组；每组12只。对照组大鼠摘除少量卵巢周围脂肪，其余各组大鼠手术摘除双侧卵巢，制备骨质疏松模型。手术后第5天，雌二醇组大鼠皮下注射雌二醇，不同剂量CDE组大鼠分别开始灌胃相应剂量的CDE，对照组和模型组大鼠给予等量蒸馏水。给药20周后，质体比法检测大鼠右侧股骨密度，骨力度仪检测大鼠左侧股骨生物力学指标，ELISA法检测大鼠血清中碱性磷酸酶（ALP）及骨钙素水平，全自动生化仪检测大鼠血清中钙离子和磷离子水平，HE染色观察大鼠子宫组织形态。结果：与模型组比较，雌二醇组及低、中和高剂量CDE组大鼠右侧股骨密度明显升高（P<0.05或P<0.01），各剂量CDE组间比较差异无统计学意义（P>0.05）；与模型组和低剂量CDE组比较，中和高剂量CDE组大鼠左侧股骨的最大弯曲力、最大应变和骨刚性明显升高（P<0.05），血清中ALP水平降低（P<0.05）。与模型组及低、中剂量CDE组比较，高剂量CDE组大鼠血清中骨钙素水平明显降低（P<0.05）。与模型组比较，低、中和高剂量CDE组大鼠血清钙离子水平明显升高（P<0.05），各剂量CDE组间比较差异无统计学意义（P>0.05）。与模型组比较，低、中和高剂量CDE组大鼠子宫组织形态表现均有不同程度改善。结论：CDE可以通过升高大鼠血清ALP、骨钙素及钙离子水平起到治疗骨质疏松的作用。

Therapeutic effect of cistanche deserticola ethanol extraction in rats with osteoporosis induced by ovariectomy and its mechanism

Objective:To explore the therapeutic effect of cistanche deserticola ethanol extraction (CDE) in the rats with osteoporosis induced by ovariectomy,and to clarify its mechanism.Methods:A total of 72 female SD rats were randomly divided into control,model,estradiol (0.3 g · kg-1),low,middle and high doses (0.5,1.0 and 2.0 mg · kg-1) of CDE groups,and 12 rats in each group.The rat ovaries were removed by operation in the other groups to establish the osteoporosis models,while the same size of fat of the rats in control group was excised.5 d later,the rats in estradiol group were hypodermicly injected with estradiol,and the rats in CDE groups were orally administrated with corresponding doses of CDE.The same volume of diluted water was given to the rats in control and model groups.After 20 weeks,the density of right femur of the rat was detected with the method of ratio of weight and size;the mechanics index of left femur of the rat was analyzed by bone granulometer;the levels of serum alkaline phosphatase (ALP) and osteocalcin were measured by ELISA;the levels of serum Ca2+ and P were detected by automatic biochemical analyzer;the morphology of uterus tissue was observed by HE staining.Results:Compared with model group,the densities of right thighbone of the rats in estradiol group and low,middle and high doses of CDE groups were significantly increased (P＜0.05 or P＜0.01);there were no differences between low,middle and high doses of CDE groups (P＞0.05).Compared with model group and low dose of CDE group,the maximum bending forces,the maximun strains,and the bone rigid coefficients in middle and high doses of CDE groups were remarkably increased (P＜ 0.05),while the levels of ALP were decreased (P＜ 0.05).Compared with model group,and low and middle doses of CDE groups,the level of osteocalcin in high dose of CDE group was significantly decreased (P＜0.05).Compared with model group,the levels of Ca2+ in serum of the rats in low,middle and high doses of CDE groups were significantly increased (P＜0.05);there were no significant differences between low,middle and high doses of CDE groups (P＞0.05).Compared with model group,the morphology of uterus tissue of the rats in low,middle and high doses of CDE groups was improved at different degrees.Conclusion:CDE has therapeutic effect on osteoporosis through increasing the levels of serum ALP,osteocalcin and Ca2+ of the rats.