结直肠癌组织中B7-H1和B7-H4表达与Foxp3+调节性T细胞浸润的关联性

目的：探讨B7-H1和B7-H4在结直肠癌（CRC）组织中的表达及其与肿瘤中Foxp3⁺调节性T细胞（Treg）浸润的关系，为判定CRC的生物学行为及预后提供依据。方法：免疫组织化学法检测56例CRC患者肿瘤标本及癌旁正常组织样本中B7-H1、B7-H4蛋白的表达和Treg的浸润情况，并与CRC患者的临床病理特征进行关联性分析。结果：CRC患者CRC组织中B7-H1阳性表达率高于癌旁正常组织（χ²=72.34，P<0.01）。不同浸润深度的CRC组织中B7-H1表达频数比较差异有统计学意义（P<0.01）；淋巴结转移阳性者CRC组织中B7-H1高表达频数高于淋巴结转移阴性者（P<0.01）；Duke's分期C+D者CRC组织中B7-H1高表达频数高于Duke's分期A+B者（P<0.01）。CRC组织中B7-H4阳性表达率高于癌旁正常组织（χ²=78.92，P<0.01）。不同浸润深度的CRC组织中B7-H4高表达频数比较差异有统计学意义（P<0.05），淋巴结转移阳性者CRC组织中B7-H4高表达频数高于淋巴结转移阴性者（P<0.05）；肿瘤组织中Treg阳性细胞数明显高于癌旁正常组织（P<0.01）；分化程度不同CRC组织中Treg阳性细胞数比较差异有统计学意义（P<0.01）；淋巴结转移阳性者CRC组织中Treg阳性细胞数高于淋巴结转移阴性者（P<0.01）。Treg浸润与B7-H1的表达有相关性（P<0.01，Cramer's=0.463），Treg浸润与B7-H4的表达有相关性（P<0.05，Cramer's=0.320）。结论：B7-H1和B7-H4在CRC组织中呈异常高表达，并与肿瘤中Treg浸润增加有关，可作为评估CRC预后的指标。

Relationships between expressions of B7-H1 and B7-H4 and Foxp3+ regulated T-cell infiltration in colorectal cancer tissue

Objective: To explore the expressions of B7-H1 and B7-H4 in colorectal cancer(CRC) tissue and their relationships with the Foxp3⁺ regulated T-cell (Treg) infiltrationin tumor tissue,and to provide the basis for judging the biological behaviours and prognosis of CRC. Methods: Immunohistochemistry was used to detect the expressions of B7-H1 and B7-H4 and the infiltration of Treg in 56 cases of CRC tissue samples and adjacent normal tissue samples,and their relationships with the clinicopathological features of CRC patients were analyzed. Results: The positive expression rate of B7-H1 in CRC tissue of the CRC patients was higher than that in adjacent normal tissue(χ²=72.34,P<0.01).The differences of expression frequencies of B7-H1 in CRC tissues with different infiltration depths were significant (P<0.01). The high expression frequency of B7-H1 in CRC tissue of the patients with positive lymphnode metastasis was higher than that in the patients with negative lymphnode metastasis(P<0.01).The high expression frequency of B7-H1 in CRC tissue of the patients at Duke's stage (C+D) was higher than that of the patients at Duke's stage (A+B)(P<0.01).The positive expression rate of B7-H4 in CRC tissue was higher than that in adjacent normal tissue(χ²=78.92,P<0.01).The differences of expression frequencies of B7-H4 in CRC tissues with different infiltration depths were significant(P<0.05).The high expression frequency of B7-H4 in CRC tissue of the patients with positive lymphnode metastasis was higher than that of the patients with negative lymphnode metastasis(P<0.05).The number of Treg positive expression cells in CRC tissue was significantly higher than that in adjacent normal tissue (P<0.01),the number of Treg postive expression cells in CRC tissues with different differentiation degrees were different(P<0.01),and the number of Treg positive expression cells in CRC tissue of the patients with positive lymphnode metastasis was higher than that of the patients with negative lymph node metastasis(P<0.01).The expression of B7-H1 was correlated with Treg infiltration(P<0.01,Cramer's=0.463);the expression of B7-H4 was correlated with Treg infiltration(P<0.05,Cramer's=0.320). Conclusion: B7-H1 and B7-H4 are highly expressed in human CRC tissue,and they are associated with the increasing of Treg infiltration;they can be used as the indicators to judge the prognosis of CRC.