姜黄素对糖尿病肾病大鼠肾脏的保护作用及其机制

目的： 探讨姜黄素对糖尿病肾病(DN)大鼠肾脏结缔组织生长因子( CTGF ) 表达和肾脏排泄功能的影响，阐明姜黄素对DN大鼠肾脏的保护作用及其机制。方法：40只雄性SD大鼠分为正常对照组(n=12)和模型组(n=28),采用腹腔注射链脲佐菌素(STZ)的方法建立DN大鼠模型,24只造模成功的大鼠随机分为模型组(n=12)和姜黄素组(n=12)。于用药8周末检测大鼠血糖水平、体质量、肾质量、肾质量/体质量、24 h尿微量蛋白清除率(UAER)和血尿素氮（BUN）、血肌酐（Scr）水平；HE染色观察大鼠肾组织病理学变化；免疫组织化学法检测肾皮质CTGF蛋白表达强度。结果： 与模型组比较，姜黄素组大鼠一般情况明显改善，空腹血糖降低（P<0.01)，体质量明显增加(P<0.01)；与模型组比较，姜黄素组大鼠肾质量、肾质量／体质量、UAER、BUN和Scr明显降低（P<0.01）；与模型组比较，姜黄素组大鼠肾组织着色程度明显减轻，CTGF表达显著下降（P<0.01）；与模型组比较，姜黄素组大鼠肾小球肥大、系膜细胞增生、肾小球变形病变明显减轻。结论：姜黄素能明显改善大鼠肾脏功能，其机制可能与抑制肾脏CTGF表达有关。

Protective effect of curcumin on kidney of rats with diabetic nephropathy and its mechanism

Objective To investigate the effects of curcumin on the expression of connective tissue growth factor(CTGF) in kidney and kidney excretion of the rats with diabetic nephropathy(DN) rats,and to clarify the protection mechanism of curcumin on the kidney of DN rats.Methods 40male SD rats were divided into normal control group(n=12) and DNmodel group(n=28). The diabetic model was induced by intraperitoneal injection of streptozotocin(STZ).24 DN model rats were randomly divided into model group(n=12) and curcumin treatment group(n=12).The blood sugar,body weight,kidney weight,kidney weight/ body weight,24 h urinary albumin excretion rate(UAER),serum creatinine (Scr),bloodnitrogen（BUN）of the rats were detected 8 weeks after treatment;the pathological changes of the rat kidney tissues was observed by HE staining,and the expression of CTGF protein was determined by immunohistochemistry.Results Compared with model group,the general satus of the rats in curcumin group was significantly improved;the fasting blood glucose was significantly decreased(P<0.01),the body weight of the rats was increased (P<0.01)，and the kidney weight,kidney weight / body weight,24 h UAER,Scr and BUN were significantly reduced(P<0.01);the staining degree of the kidnry tissue was significantly decreased,the expression of CTGF protein was significantly alleviated in curcumin group(P<0.01);glomerular hypertrophy,mesangial cell proliferation and glomerular lesions were significantly alleviated.Conclusion Curcumin can obviously improve the kidney function,and its mechanism may be correlated with inhibition of CTGF expression in kindey.