甲基汞对小鼠睾丸生殖细胞周期的影响 |
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引用本文: | 金明华,石龙,孙志伟,胡颖,刘晓梅,宋军学,刘倪.甲基汞对小鼠睾丸生殖细胞周期的影响[J].吉林大学学报(医学版),2002,28(5):465-466. |
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作者姓名: | 金明华 石龙 孙志伟 胡颖 刘晓梅 宋军学 刘倪 |
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作者单位: | 1. 吉林大学公共卫生学院卫生化学教研室,吉林,长春,130021;2. 吉林大学第一医院干部病房 |
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基金项目: | 国家自然科学基金;39570607; |
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摘 要: | 目的:研究甲基汞对小鼠睾丸生殖细胞周期的影响.方法:采用流式细胞术技术观察小鼠睾丸生殖细胞周期的变化.结果:随着甲基汞染毒剂量的增加,G0/G1期细胞没有明显变化;S时相细胞的百分数逐渐减少,甲基汞1/50 LD50和1/10 LD50组S时相细胞百分数与阴性对照组比较差异有显著性(P<0.05);G2/M时相细胞百分数则显著增加,各染毒剂量组与阴性对照组比较差异有显著性(P<0.05).结论:不同剂量的甲基汞可以引起S时相细胞百分数减少,DNA合成受到抑制,并出现G2期细胞阻滞.
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关 键 词: | 甲基汞 睾丸 生殖细胞 细胞周期 小鼠 |
文章编号: | 1671-587X(2002)05-0465-02 |
修稿时间: | 2002年1月15日 |
The effect of methyl mercury on the cell cycles of germ cells in the mouse testis |
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Abstract: | Objective: To study on the effects of methyl mercury (MeHg) on the cell cycles of the mouse testis germ cells. Methods: The flow cytometry was used to examine the cell cycles of the mouse testis germ cells. Results: Cell percentage had not significantly changed in G 0/G 1 but decreased in S phase with the increasing dosage of MeHg. The 1/50 LD 50 and 1/10 LD 50 also have significantly changed in S phase compared with that of negative control ( P <0.05). The cell percentage trearted with MeHg were significantly higher in G 2/M phase than that of the control( P <0.05). Conclusion: MeHg may decrease cell percentage in S phase, inhibit the DNA synthesis and make cell block in G 2 phase. |
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Keywords: | methyl mercury testis germ cells cell cycle mice |
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