20(S)-人参皂苷Rg3抑制肿瘤生长的作用

目的：观察20(S)-人参皂苷Rg3的抗肿瘤生长的作用并探讨其机制。方法：通过B16黑色素瘤实体瘤模型、MTT法观察Rg3对B16黑色素瘤生长的作用，采用新生血 管计数实验、流式细胞术检测Rg3对肿瘤组织血管生成及细胞周期的影响。结果：实体瘤模型中，不同浓度Rg3各组瘤重明显低于对照组（P<0.01），且Rg3组肿瘤周围的血管数也明显少于对照组（P<0.01）。体外实验中，MTT法见Rg3抑制B16黑色素细胞的生长，其IC₅₀为（7.76±0.46）mg·L^-1，并且Rg3可阻滞B16黑色素瘤细胞于G0/G1和S期，使G₂/M期细胞数明显减少。 结论：Rg3抑制B16黑色素瘤的生长，其机制可能是通过抑制肿瘤内血管生成及阻滞肿瘤细胞进入分裂期来发挥作用的。

Inhibitory effect of 20 (S) -ginsenoside Rg3 on tumor growth

Objective To study the inhibitory effect of 20 (S) -ginsenoside Rg3 on tumor growth and its mechanism. Methods MTT assay and the model of B16 melanoma were used to observe the effect of 20 (S) -ginsenoside Rg3 on B16 melanoma growth and its mechanism was analysed by assay of tumor-induced angiogenesis and flow cytometry. Results In vivo, the average weight of B16 melanoma in each Rg3 group was lower than that in control group (P<0. 01) and the number of vesssels oriented toward the tumor mass was less than that in untreated control (P<0. 01). In vitro, the results of MTT assay displayed that ginsenoside Rg3 inhibited the growth of B16 melanoma cells and the IC50 value was (7. 76±0. 46) mg · L~1. Rg3 blocked B16 melanoma cells in G0/G1 and S stages. Conclusion 20 (S) -ginsenoside Rg3 can obviously inhibit the tumor growth and the effect may be related to its inhibiting angiogenesis and blocking cell mitosis.