香菇多糖对电离辐射所致小鼠损伤的保护作用

目的：探讨香菇多糖（PLE）对辐射损伤小鼠免疫、抗氧化及造血功能损伤的影响。方法：50只ICR小鼠随机分为正常对照组（NC）、辐射对照组（IC）和PLE低、中、高剂量给药组，剂量分别为 800、1 600 和2 400 mg?kg-1，连续7 d灌胃给药，NC组和IC组给予等量的生理盐水。第8天除NC组外，均接受2.0 Gy X线照射，检测小鼠脾指数和胸腺指数、过氧化物歧化酶（SOD）活性、肝脏丙二醛（MDA）含量和照射24 h后小鼠外周血白细胞数和骨髓嗜多染红细胞(PCE)微核数。 结果：与IC组比较，PLE高剂量组脾指数、胸腺指数、小鼠肝脏SOD活性明显升高（P< 0.05），PLE中、高剂量组小鼠肝脏中MDA的含量明显升高（P< 0.05），PLE各剂量组小鼠白细胞数明显升高（P< 0.05），中、高剂量组小鼠骨髓PCE微核数明显降低（P< 0.05）。结论：PLE对电离辐射所致的小鼠损伤有明显的保护作用。

Protective effect of polysaccharides of lentinus edodes on damage in mice induced by ionizing radiation

Objective To evaluate the protective effect of polysaccharides of lentinus edodes (PLE) on immune and anti-oxidative and hematopoietic function of ionizing irradiated mice,and provide theoretical basis for its chinical application.Methods Fifty ICR mice were randomly divided into normal control group(NC),irradiating control group(IC),low dose of PLE irradiating group(800 mg·kg-1),middle dose of PLE irradiating group(1 600 mg·kg-1),and high dose of PLE irradiating group(2 400 mg·kg-1)(n=10).The mice in PLE groups were gaster-poured by PLE for 7 d,the mice in NC group and IC group were gaster-poured by 0.9% sodium chloride solution. The mice in all groups except NC group were irradiated with 2 Gy X-ray on the eighth day.The spleen index,thymus index,the activity of SOD and content of MDA,the number of WBC,the micronucleus rate of bone marrow polychromalic erythrocytes (PCE)were detected at the 24th hour after radiation.Results Compared with IC group,the spleen index,thymus index and activity of SOD in high dose of PLE irradiating group were increased markedly(P<0.05); the contents of MDA in middle and high dose of PLE irradiating groups were increased significantly(P<0.05).The number of WBC in PLE groups was increased significantly (P<0.05);the micronucleus rates of bone marrow PCE in middle and high dose of PLE irradiating groups were decreased significantly (P<0.05).Conclusion PLE has an obvious protective effect on damage in X-ray irradiated mice.