先天性白内障大鼠晶状体中二肽基肽酶Ⅱ免疫活性分析

目的：探讨二肽基肽酶Ⅱ (DPPⅡ)与先天性白内障发病的关系。方法：先天性白内障大鼠(SCR)80只，随机均分为8、10、12及14周龄组。以各同周龄正常Wistar大鼠作为正常对照组。应用免疫组织化学法(ABC染色法)，即抗生物素蛋白-生物素-过氧化物酶复合体法,观察8、10、12及14周龄SCR晶状体中DPPⅡ的免疫活性变化。结果：8、10、12及14周龄SCR,白内障晶状体上皮细胞及周边部晶状体纤维组织中，DPPⅡ染色较同周龄正常对照组均明显增强,特别是12及14周龄SCR白内障晶状体中DPPⅡ的免疫阳性产物扩展至晶状体核周部。结论：DPPⅡ在先天性SCR晶状体中免疫活性增强,它有可能在白内障形成过程中参与晶状体蛋白的水解分化,从而促进白内障的形成。

Immunoreactivity changes of dipeptidyl peptidase Ⅱ in lens in SCR rats with congenital cataract

Objective To explore the relationship between dipeptidyl peptidaseⅡ(DPPⅡ) and the pathogenesis of congenital cataract.Methods Eighty SCR rats with congenital cataract were randomly divided into four groups:8,10,12 and 14 weeks groups(n=20).Normal Wistar rats at each age were used as control groups.Immunohistochemical experiments using the streptavidin-biotin immunoper-oxidase method were used to observe the immunoreactivity changes of DPPⅡ in lens in SCR rats at different time(8,10,12,14 weeks).Results The immunoreactivities of DPPⅡ in the lens epithelial cells and fibres of cataractous lenses of all ages were higher than those in control groups.At the 12th and 14th week,immunoreactive staining of DPPⅡwas found to extend into the perinuclear region.Conclusion The immunoreactivity of DPPⅡ is enhanced in cataractous rat lenses.DPPⅡ may be participated in the proteolytic modification of lens proteins during cataractogenesis.