前列舒通片对鼠的抗炎及镇痛作用

目的：观察前列舒通片（QLS）对实验性动物一般炎症与镇痛模型的作用，为临床应用提供药效学依据。方法：大鼠和小鼠分别均按体重区组随机方法分为空白对照组（n=8）、阳性对照组（阿司匹林组，n=8）、低剂量组（小鼠：25 mg?kg^-1?d^-1，n=8；大鼠： 17.5 mg?kg^-1?d^-1，n=8）、QLS中剂量组（小鼠：50 mg?kg^-1?d^-1，n=8；大鼠：35.0 mg?kg^-1?d^-1，n=8）、QLS高剂量组（小鼠：100 mg?kg^-1?d^-1，n=8；大鼠：70.0 mg?kg^-1?d^-1，n=8）。QLS各剂量组均连续灌胃给药3 d。采用二甲苯致小鼠耳肿胀、卵白蛋白致大鼠足肿胀和棉球肉芽肿观察其对炎症反应的影响，并采用小鼠热板法和腹腔注射0.6 %醋酸扭体法观察其镇痛作用。结果：QLS 3个剂量组均不同程度地抑制小鼠耳肿胀度，与空白对照组比较，50及100 mg?kg^-1?d^-1 QLS组小鼠耳肿胀度均明显减小（P<0.05或P<0.01），对小鼠耳肿胀的抑制率分别为53.97%和60.09%；QLS 35.0及70.0 mg?kg^-1?d^-1组均显著抑制大鼠足肿胀（P<0.05），且对棉球所致肉芽组织增生有明显的抑制作用（P<0.05或P<0.01），大鼠肉芽肿的抑制率分别为32.40 %和35.65 %。与空白对照组比较， QLS 25、50及100 mg?kg^-1?d^-1组分别在给药后90、90及30 min均明显提高热板法小鼠的痛阈值（P<0.05或P<0.01），且可明显减少小鼠的扭体次数（P<0.05），对小鼠痛阈抑制率分别为36.17%、44.13%和46.64%。结论：QLS具有明显的抗炎和镇痛作用。

Anti-inflammatory effects and analgesic effects of Qianlie Shutong Tablets on rats or mice

Objective To investigate anti-inflammatory effects and analgesic effects of Qianlie Shutong Tablets(QLS) and provide medical basis for its clinical use.Methods A total of 40 rats or mice were randomly assigned into control group(n=8),drug control(Aspirin,n=8) group,low-dose QLS(mice: 25 mg·kg-1·d-1,n=8;rats: 17.5 mg·kg-1·d-1,n=8) group,mid-dose QLS(mice: 50 mg·kg1·d-1,n=8;rats: 35.0 mg·kg-1·d-1,n=8) group and high-dose QLS(mice: 100 mg·kg-1·d-1;rats: 70.0 mg·kg-1·d-1) group.Anti-inflammatory effects were tested by xylene-induced ear edema in mice,ovoalbumin-induced feet edema and cotton pellet-induced granuloma in rats.Analgesic effects were tested by hot-plate method and 0.6 % acetic acid solution injected into the mice abdominal cavity to produce pain in order to cause the body bend.Different doses of QLS were given and their actions were observed.Results QLS had obvious anti-inflammatory effectts on the xylene-induced ear edema.Compared with control group,ear edema was significantly decreased by QLS with the doses of 50 and 100 mg·kg-1·d-1(P<0.05 or P<0.01),the ear edema inhibitory rates were 53.97 % and 60.09 % in mice,respectively.Ovoalbumin-induced rat feet edema was significantly decreased by QLS with the doses of 35 and 70.0 mg·kg-1·d-1 (P<0.05).Cotton pellet-induced rat granuloma was significantly decreased by QLS with the doses of 35.0 and 70.0 mg·kg-1·d-1(P<0.05 or P<0.01) and the granuloma inhibitory rates were 32.40 % and 35.65 %,respectively.Pain thresholds 90,90 and 30 min after administration were markedly elevated(P<0.05 or P<0.01) and the licking-paw latencies in hot plate method were extended by QLS with the doses of 25,50 and 100 mg·kg-1·d-1(P<0.05),the inhibitory rates of pain threshold were 36.17%,44.13%,and 46.64%,respectively.Conclusion QLS has significant anti-inflammatory and analgesic effects.