四妙勇安汤对大鼠血栓闭塞性脉管炎的抗炎作用及其机制

目的：观察四妙勇安汤（SMYA）对血栓闭塞性脉管炎（TAO）模型大鼠的治疗作用，并探讨其作用机制。方法：将实验大鼠随机分为假手术组、模型组、脉络宁阳性对照组（2.7 g/kg）及SMYA高、中、低剂量组（24.3、16.2和8.1 g/kg）。采用大鼠股动脉注射月桂酸钠法建立大鼠TAO模型，造模后灌胃给药，以脉络宁颗粒（MLN）为阳性对照药，连续给药15 d后处死动物取材，常规HE染色观察大鼠血管及周围组织的病理学变化，采用酶联免疫吸附法（ELISA）检测血浆中白细胞介素6（IL-6）、肿瘤坏死因子α（TNF-α）、C-反应蛋白（CRP）、血栓素B2(TXB2)和6-酮-前列腺素F1α(6-K-PGF1α)含量，并应用紫外分光光度计测量血浆中一氧化氮（NO）的含量。结果：与模型组比较，SMYA高剂量组大鼠血浆中IL-6、TNF-α、TXB2和CRP含量明显降低，NO和6-K-PGF1α含量明显增加，差异均有统计学意义（P<0.05或P<0.01）；SMYA中剂量组和低剂量组大鼠血浆中TNF-α含量明显降低（P<0.01）。模型组大鼠血管内皮受损严重，周围有大量炎细胞浸润；SMYA高剂量组大鼠血管皮细胞基本完整，有少量炎细胞浸润。结论：SMYA对TAO模型大鼠具有较好的治疗作用，其机可能与抗炎和维持血浆中血栓素A2(TXA2)和前列环素I2(PGI2)平衡功能有关。

Anti-inflammatory effect of Simiaoyong' an Decoction in thromboangiitis obliterans rats and its mechanism

Objective To observe the therapeutic effect of Simiaoyong'an Decoction(SMYA) on the rat models with thromboangiitis obliterans(TAO),and to explore the action mechanism.Methods The rats were randomly divided into 6 groups:sham operation group,model group,mailuoning group(MLN 2.7 g·kg-1),high,medium and low doses of SMYA groups(24.3,16.2,8.1 g·kg-1).The TAO model was prepared by injecting sodium laurate into the femoral artery of rats.The rats were administered by intragastric administration after prepared model.After 15 d administration,the pathological changes of blood vessel and surrounding tissue were observed with HE staining,the contents of IL-6,TNF-α,CRP,TXB2 and 6-K-PGF1α in plasma were measured by enzyme-linked immunosorbent assay(ELISA),and the content of NO in plasma was measurd by ultraviolet spectrophotometer.Results Compared with model group,the contents of plasma IL-6,TNF-α,TXB2 and CRP in high dose of SMYA(24.3 g·kg-1) group were significantly decreased,and the contents of NO and 6-K-PGF1α were markedly increased(P<0.05 or P<0.01);the contents of plasma TNF-α in medium and low doses of SMYA(16.2 and 8.1 g·kg-1) groups were significantly decreased(P<0.01).The vascular endothelial cells in model group were badly damaged with a large number of inflammatory cells infiltration,and the vascular endothelial cells in high dose of SMYA group showed integrity with little inflammatory cells infiltration.Conclusion SMYA has a good therapeutic effect on rat model of TAO,and the mechanism may be related to anti-inflammatory and maintaining the balance function of TXA2 and PGI2 in plasma.