慢性乙肝病毒感染各阶段患者外周血单个核细胞中IL-22mRNA的表达水平及其临床意义

目的: 分析慢性乙型病毒性肝炎(CHB)、慢加急性/亚急性重型肝炎(重型肝炎)、乙肝肝硬化和乙型肝炎病毒(HBV)相关性原发性肝癌(HCC)患者外周血单个核细胞(PBMCs)中白细胞介素22(IL-22)mRNA的表达水平及其可能的临床意义。方法: 收集20名健康对照者(健康对照组)、33例CHB中度患者(CHB中度组)、21例CHB重度患者(CHB重度组)、16例重型肝炎患者(重型肝炎组)、16例乙肝肝硬化患者(肝硬化组)和40例HCC患者(HCC组)的临床资料。RT-PCR半定量分析各组研究对象PBMCs中IL-22mRNA表达水平;Pearson相关分析法分析IL-22mRNA表达水平与临床重要检测指标的相关性。结果: CHB中度、CHB重度和肝硬化组患者PBMCs中IL-22mRNA表达水平均高于健康对照组(P<0.001);重型肝炎组患者PBMCs中IL-22mRNA表达水平与健康对照组比较差异无统计学意义(P=0.830);HCC组患者PBMCs中IL-22mRNA表达水平明显低于健康对照组(P<0.001)。CHB重度组患者和重型肝炎组患者PBMCs中IL-22mRNA表达水平与总胆红素(TB)水平呈明显负相关关系(r=-0.383,P=0.043;r=-0.619,P=0.028);HCC组患者PBMCs中IL-22mRNA表达水平与血清甲胎蛋白(AFP)水平呈明显正相关关系(r=0.664,P=0.000)。结论: 慢性乙肝重症化过程中,患者PBMCs中IL-22mRNA表达水平低下提示肝细胞再生障碍,且IL-22mRNA表达水平与TB一样能够反映患者病情严重程度。IL-22可能对HCC的发生发展起促进作用。

Expression levels of IL-22 mRNA in PBMCs of patients at different HBV infection phases and their significances

Objective: To detect the expression levels of IL-22 mRNA in peripheral blood mononuclear cells (PBMCs) of the patients with chronic hepatitis B (CHB),acute/subacute-on-chronic liver failure (severe hepatitis),and hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC),and to investigate their clinical significances. Methods: A total of 20 healthy controls (control group),33 patients with moderate CHB(moderate CHB group),21 patients with severe CHB(severe CHB group),16 patients with severe hepatitis(severe hepatitis group),16 patients with cirrhosis (cirrhosis group) , and 40 patients with HCC(HCC group) were enrolled.The expression levels of IL-22 mRNA in PBMCs of the subjects in various groups were tested by RT-PCR method;Pearson correlation analysis was used to analyze the relationship between the IL-22 mRNA expression levels and the important clinical indexes. Results: The expression levels of IL-22 mRNA in PBMCs of the patients in moderate CHB,severe CHB and cirrhosis groups were all higher than that in control group(P<0.001);there were no significant differences of the expression levels of IL-22 mRNA of the subjects between severe hepatitis group and control group (P=0.83);the expression level of IL-22 mRNA in PBMCs of the patients in HCC group was lower than that in control group (P<0.001);the expression level of IL-22 mRNA in PBMCs was negatively correlated with the serum total bilirubin(TB) level in severe CHB and severe hepatitis groups(r=-0.383,P=0.043;r=-0.619,P=0.028);the expression level of IL-22 mRNA was positively correlated with the serum alpha-fetoprotein (AFP) level in HCC group (r=-0.664,P=0.000). Conclusion: The decreasing of IL-22 mRNA expression level in the patients with deteriorative hepatitis suggests the dysfunction of liver regeneration;the IL-22 mRNA expression level,as TB,can reflect the severity of liver injury. IL-22 may have promotion effect on the occurrence and development of HCC.