SENP1、SENP2和SENP6蛋白在人恶性胶质瘤组织和细胞中的表达及其意义

目的：观察SUMO特异性蛋白酶（SENPs）家族成员SENP1、SENP2和SENP6在人恶性胶质瘤组织和细胞中的表达，阐明其在恶性胶质瘤发生中的作用。方法：取人脑正常组织和恶性胶质瘤组织样品分别作为正常对照组和恶性胶质瘤组；培养Cos7细胞和恶性胶质瘤LN443和U343细胞，Cos7细胞作为正常细胞对照组，LN443和U343作为恶性胶质瘤细胞组；采用Western blotting法检测SENP1、SENP2和SENP6蛋白在人恶性胶质瘤组织和细胞中的表达水平。结果：在脑组织中，与正常对照组比较，恶性胶质瘤组恶性胶质瘤组织中SENP1、SENP2和SENP6蛋白表达水平均明显升高（P<0.05）。在细胞中，与正常细胞对照组比较，恶性胶质瘤细胞组LN443和U343细胞中SENP1、SENP2和SENP6蛋白表达水平均明显升高（P<0.05）。结论：SENP1、SENP2和SENP6在恶性胶质瘤组织和细胞中高表达，其可能对恶性胶质瘤的发生起到了重要的促进作用。

Expressions of SENP1,SENP2 and SENP6 proteins in human glioma tissue and cells and their mechanisms

Objective: To observe the expressions of SENP1, SENP2 and SENP6 proteins in human malignant glioma tissue and cells, and to elucidate the their effects in the development of malignant glioma.Methods: The samples of normal human brain tissue and malignant glioma tissue were obtained and used as normal control group and malignant glioma group, respectively.The Cos7 cells and the malignant glioma LN443 and U343 cells were cultured;the Cos7 cells were used as normal cell control group,and the LN443 and U343 cells as malignant glioma cell group. Western blotting method was used to detect the expression levels of SENP1, SENP2 and SENP6 proteins in human malignant glioma tissue and cells.Results: In brain tissue, the expression levels of SENP1, SENP2 and SENP6 proteins in malignant glioma group were higher than those in normal control group(P<0.05). Compared with normal cell control group, the expression levels of SENP1, SENP2 and SENP6 proteins in the LN443 and U343 cells in malignant glioma cell group were significantly increased (P<0.05).Conclusion: SENP1, SENP2 and SENP6 proteins highly express in the malignant glioma tissue and cells, and they may play an important role in promoting the occurrence of malignant glioma.