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重组MUC1-MBP融合蛋白联合R848对小鼠T细胞免疫活性的诱导作用
引用本文:孙敏英,刘果木,接晶,谢飞,翟瑞萍,陈潭秀,袁红艳,台桂香.重组MUC1-MBP融合蛋白联合R848对小鼠T细胞免疫活性的诱导作用[J].吉林大学学报(医学版),2017,43(3):507-511.
作者姓名:孙敏英  刘果木  接晶  谢飞  翟瑞萍  陈潭秀  袁红艳  台桂香
作者单位:吉林大学基础医学院医学生物学实验中心,吉林 长春,130021;吉林大学基础医学院免疫学教研室,吉林 长春,130021
基金项目:吉林省科技厅科技攻关双十工程项目资助课题,吉林省长春市科技局科技项目资助课题
摘    要:目的:研究MUC1-MBP联合TLR7/8激动剂R848对小鼠T细胞免疫活性的诱导作用,为寻找重组MUC1-MBP融合蛋白的合适佐剂提供实验依据。方法:21只C57BL/6小鼠随机分为对照组(注射生理盐水)、MUC1-MBP+ R848组(注射MUC1-MBP+ R848)和MUC1-MBP+卡介苗(BCG)组(注射MUC1-MBP+ BCG),每组7只。第2次免疫后第4~7天无菌取脾脏组织,测定脾指数;淋巴细胞增殖反应测定各组小鼠的刺激指数(SI);ELISA法测定小鼠脾细胞培养上清中肿瘤坏死因子γ(TNF-γ)和 白细胞介素4(IL-4)水平;流式细胞术检测小鼠脾细胞中T淋巴细胞亚群比例。结果:与对照组比较,MUC1-MBP+R848组和MUC1-MBP+BCG组小鼠脾指数均升高(P<0.01),SI升高(P<0.05),TNF-γ水平升高(P<0.05或P<0.01);且MUC1-MBP+R848组小鼠上述指标高于MUC1-MBP+BCG组(P<0.05或P<0.01);IL-4水平略微升高,但差异无统计学意义(P>0.05)。与对照组比较,MUC1-MBP+ R848组和MUC1-MBP+ BCG小鼠脾细胞中CD3+细胞、CD4+细胞和CD8+细胞比例明显升高(P<0.05或P<0.01)。结论:MUC1-MBP 融合蛋白联合R848或BCG均能诱导小鼠倾向于Th1的细胞免疫应答反应,R848是良好的MUC1-MBP候选佐剂分子。

关 键 词:MUC1-MBP  R848  肿瘤疫苗  佐剂
收稿时间:2016-09-19

Inductive effect of recombinant MUC1-MBP fusion protein combined with R848 on immune activity of T cells in mice
SUN Minying,LIU Guomu,JIE Jing,XIE Fei,ZHAI Ruiping,CHEN Tanxiu,YUAN Hongyan,TAI Guixiang.Inductive effect of recombinant MUC1-MBP fusion protein combined with R848 on immune activity of T cells in mice[J].Journal of Jilin University: Med Ed,2017,43(3):507-511.
Authors:SUN Minying  LIU Guomu  JIE Jing  XIE Fei  ZHAI Ruiping  CHEN Tanxiu  YUAN Hongyan  TAI Guixiang
Institution:1. Experimental Center of Medical Biology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China;
2. Department of Immunology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China
Abstract:Objective:To study the inductive effect of recombinant MUC1-MBP fusion protein combined with R848 on the immune activity of T cells in the mice,and to provide experimental basis for searching the suitable adjuvants of recombinant MUC1-MBP fusion protein.Methods:A total of 21 C57BL/6 mice were randomly divided into control group(treat with normal saline),MUC1-MBP+R848 group (treated with MUC1-MBP+R848),and MUC1-MBP+baillus calmette guerin(BCG) group(treated with MUC1-MBP+BCG)(n=7).After immunized for 4-7 d,the spleen tissue was taken and the spleen indexes of the mice in various groups were measured;the stimmulus index(SI) of the mice was detected by lymphocyte proliferation response;the levels of tumor necrosis factor-γ(TNF-γ) and interleukin-4(IL-4) were detected by ELISA method;the proprotions of T lymphocyte subsets in spleen cells were analyzed by flow cytometry.Results:Compared with control group,the spleen indexes,SI,and TNF-γ levels of the mice in MUC1-MBP+ R848 and MUC1-MBP+BCG groups were significantly increased (P<0.05 or P<0.01);the indexes metioned above of the mice in MUC1-MBP+ R848 were higher than those in MUC1-MBP+BCG group;the IL-4 levels had no significant difference(P>0.05).Compared with control group,the proprotions of CD3+T,CD4+T,and CD8+ T cells of the mice in MUC1-MBP+ R848 group and MUC1-MBP+BCG group were significantly increased(P<0.05 or P<0.01).Conclusion:Both MUC1-MBP fusion protein combined with R848 and BCG can induce the Th1 type of immune activity in the mice,and R848 is the potential candidate adjuvant for MUC1-MBP.
Keywords:MUC1-MBP  R848  cancer vaccine  adjuvant
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