支气管哮喘患者外周血中白细胞介素22水平的检测及其临床意义

目的：检测支气管哮喘患者外周血中白细胞介素22（IL-22）水平，分析其与免疫球蛋白E（IgE）、肺功能和呼出气一氧化氮（FENO）的关系，探讨IL-22在支气管哮喘发病中的作用。方法：选择78例支气管哮喘患者作为实验组，根据血浆IgE水平将支气管哮喘患者分为IgE升高组（n=46）和IgE正常组（n=32）；根据患者的临床症状将支气管哮喘患者分为急性发作期和临床缓解期；另外选择20名健康人作为对照组。检测各组受试者外周血中IL-22、IgE水平及第1秒用力呼气容积（FEV1）和FENO，比较各组受试者外周血中IL-22水平、FEV1和FENO水平的变化，并分析外周血中IL-22水平与IgE、FEV1和FENO水平的相关性。结果：哮喘组患者外周血血浆IL-22和FENO水平高于对照组（P<0.05），虽然临床缓解期患者IL-22和FENO水平低于急性发作期，但仍高于对照组（P<0.05）。IgE升高组患者血浆IL-22水平高于IgE正常组（P<0.05）。急性发作期和临床缓解期支气管哮喘患者IL-22水平与FEV1呈负相关关系（r=-0.3653，P<0.05；r=-0.2672，P<0.05），与FENO无相关关系（r=0.0119，P>0.05；r=0.0598，P>0.05）。结论：IL-22在支气管哮喘患者外周血中高表达，提示其可能参与支气管哮喘的发病机制，并可能加剧肺功能恶化。

Detection of interleukin-22 in peripheral blood of patients with bronchial asthma and its clinical significance

Objective:To detect the interleukin-22(IL-22) levels in peripheral blood of the asthma patients and analyze the relationship between the IL-22 levels and IgE,pulmonary function,fractional exhaled nitric oxide(FENO),and to investigate the role of IL-22 in the pathogenesis of bronchial asthma. Methods:Seventy-eight patients with asthma were selected as experiment group.The asthma patients were divided into increased IgE group (n=46)and normal IgE group(n=32)according to the IgE levels.The asthma patients were also divided into acute attack stage and clinical control stage according to patients' clinical symptoms.In addition, twenty healthy controls were chosen as healthy control group.The plasma IL-22 levels, IgE,forced expiratory volume in one second (FEV1) and FENO levels of the subjects in various groups were detected and compared between various groups.The correlations between the plasma IL-22 levels and IgE, FEV1,FENO were analyzed.Results:The plasma IL-22 level and FENO level of the patients in asthma group were higher than those in healthy control group(P<0.05).The IL-22 level and FENO level of the patients in clinical control stage were lower than those in acute attack stage,but they were still higher than those in healthy control group(P<0.05).The plasma IL-22 level of the patients in increased IgE group was higher than that in normal IgE group(P<0.05).There were significant negative correlations between the IL-22 levels and FEV1 in the asthma patients in acute attack group and clinical control group(r=-0.365 3,P<0.05;r=-0.267 2,P<0.05),but there was no significant correlation between the IL-22 levels and FENO(r=0.011 9,P>0.05;r=0.059 8,P>0.05).Conclusion:The increased expression of IL-22 in peripheral blood of the patients with asthma may participate in the pathogenesis of asthma,and may aggravate the deterioration of pulmonary function of the asthma patients.