氯化甲基汞对NB4和K562白血病细胞凋亡及增殖的影响

目的：研究氯化甲基汞（MMC）对NB4和K562白血病细胞凋亡及增殖的影响。方法：NB4、K562细胞经1.25、2.50、5.00、10.00和20.00 μmol•L^-1MMC、三氧化二砷（ATO）分别作用0、6、12、24、48和72 h，采用MTT法测定细胞的存活率，采用Hoechst 33258染色后荧光镜下观察细胞凋亡情况。结果： NB4和K562细胞接触10 μmol•L^-1 MMC 24 h后，细胞存活率分别为22.0%和70.0%，与对照比较差异有显著性（P值分别为0.000和0.014）；NB4和K562细胞接触10 μmol•L^-1ATO 24 h后，细胞存活率分别为24.4 %和52.0%（P值分别为0.001和0.000）；荧光镜检呈现明显的凋亡图像。MMC与ATO对NB4、K562细胞诱导凋亡及抑制增殖作用相似。结论：MMC能诱导NB4和K562细胞凋亡，抑制其增殖，并呈时间剂量依赖性。

Effect of methylmercury chloride on proliferation and apoptosis of leukemia cell lines NB4 and K562

Objective To study the effect of methylmercury chloride(MMC) on proliferation and apoptosis in leukemia cell lines NB4 and K562.Methods NB4 and K562 were treated with 1.25,2.50,5.00,10.00 and 20.00 μmol·L-1 MMC and asenic troxide(ATO) for 0,6,12,24,48 and 72 h,the cell viability rate was detected by MTT assay;the apoptosis was observed with fluorescence microscope after stained by Hoechst 33258. Results The viability rates of NB4 and K562 cells treated with 10 μmol·L-1 MMC for 24 h were 22.0% and 70.0%;when treated with 10 μmol·L-1 ATO for 24 h the viability rates were 24.4 % and 52.0%;compared with control group,there was significant difference(P<0.05).The significant apoptostic picture was obtained with fluorescence microscope.The two drugs had the similar effect when they were used to treat NB4 and K562 cells in the same dose and for the same time.Conclusion MMC can induce apoptosis of NB4 and K562 cells and inhibit proliferation of leukemia cell lines NB4 and K562 significantly and the effect is in time-and dose-dependent manner.