| 辐射诱导表达载体pshuttle-Egr1-shTRAIL对肝癌SMMC7721细胞生长的抑制作用 |
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| 引用本文： | 陈志勇,刘敏,龚守良,董丽华.辐射诱导表达载体pshuttle-Egr1-shTRAIL对肝癌SMMC7721细胞生长的抑制作用[J].吉林大学学报(医学版),2011,37(6):981-984. |
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| 作者姓名： | 陈志勇 刘敏 龚守良 董丽华 |
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| 作者单位： | 内蒙古民族大学附属医院肿瘤科,内蒙古通辽028000;吉林大学第一医院肿瘤中心放疗科,吉林长春130021;吉林大学第一医院肿瘤中心放疗科,吉林长春,130021;吉林大学公共卫生学院卫生部放射生物学重点实验室,吉林长春,130021 |
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| 基金项目： | 国家自然科学基金资助课题(30570546) |
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| 摘 要： | 目的:研究携带可溶性人肿瘤坏死因子相关凋亡诱导配体(shTRAIL)基因的辐射诱导表达载体pshuttle-Egr1-shTRAIL在人肝癌细胞株中的表达及其促凋亡作用.方法:体外通过脂质体转染SMMC7721细胞.转染细胞分别接受0(假照组)、2、5和10 Gy X线照射,ELISA法检测sTRAIL的表达；细胞分为...
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| 关 键 词： | 肿瘤坏死因子相关凋亡诱导配体 基因克隆 肝肿瘤 辐射 早期生长反应1 |
| 收稿时间： | 2011-06-08 |
Inhibitory effects of recombinant plasmid pshuttle-Egr1-shTRAIL transfection in combination with X-irradiation on growth of liver cancer cells SMMC7721 |
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| CHEN Zhi-yong,LIU Min,GONG Shou-liang,DONG Li-hua.Inhibitory effects of recombinant plasmid pshuttle-Egr1-shTRAIL transfection in combination with X-irradiation on growth of liver cancer cells SMMC7721[J].Journal of Jilin University: Med Ed,2011,37(6):981-984. |
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| Authors: | CHEN Zhi-yong LIU Min GONG Shou-liang DONG Li-hua |
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| Institution: | （1. Department of Oncology,Affiliated Hospital of Innermongol Nation University,Tongliao 028000,China；2. Department of Radiotherapy,Tumor Center,First Hospital,Jilin University,Changchun130021,China；3. Key Laboratory of Radiobiology,Ministry of Health,School of Public Health,Jilin University,Changchun 130021,China） |
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| Abstract: | Objective To investigate the effects of recombinant plasmid pshuttle-Egr1-shTRAIL stable transfection in combination with X-ray irradiation on the TRAIL protein expression and the apoptosis in human SMMC7721 hepatoma cells.Methods The pshuttle-Egr1-shTRAIL packaged with liposome was stably transfected into SMMC7721 cells in vitro.The shTRAIL protein expressions were measured with ELISA assay,Annexin V-FITC kit was adopted to measure the apoptosis of pshuttle-Egr1-shTRAIL cells,and the changes in survival rate of SMMC7721 cells were measured with cell cloning assay.Results The TRAIL protein expressions in pshuttle-Egr1-shTRAIL plus different doses of irradiation groups were significantly increased compared with 0 Gy group(P< 0.001).The percentage of apoptotic cells was significantly higher than that in 0 Gy group(P<0.05 or P<0.001),and the survival rate of SMMC7721 cells was decreased significantly(P<0.05 or P<0.001).Conclusion The pshuttle-Egr1-shTRAIL stable transfection in combination with irradiation can significantly induce the apoptosis of SMMC7721 tumor cells and inhibit the cell proliferation. |
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| Keywords: | pshuttle-Egr1-shTRAIL gene clone human hepatoma SMMC7721 irradiation gene therapy |
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