柴胡疏肝散对抑郁症大鼠行为学和海马神经元凋亡及自噬的影响

目的：观察柴胡疏肝散对抑郁症模型大鼠海马神经元凋亡和自噬的拮抗作用，探讨柴胡疏肝散抗抑郁症的作用机制。方法：60只雄性成年SD大鼠随机分为正常对照组、模型组和给药组。通过给予慢性不可预见温和应激建立抑郁症模型；对照组和模型组给予同体积生理盐水，给药组在模型基础上给予柴胡疏肝散饮片溶液。采用糖水偏好实验、悬尾实验和Morris水迷宫实验进行行为学检测，流式细胞术检测大鼠海马神经元凋亡，Western blotting法检测自噬微管相关蛋白轻链3(LC-3)和自噬基因Beclin-1蛋白的表达。结果：与对照组比较，模型组和给药组大鼠糖水偏好百分比降低（P<0.05），悬尾不动时间和逃避潜伏期延长（P<0.05），细胞凋亡率、LC-3Ⅱ/LC-3Ⅰ比值和Beclin-1蛋白表达水平升高（P<0.05）；与模型组比较，给药组大鼠糖水偏好百分比升高（P<0.05），悬尾不动时间和逃避潜伏期缩短（P<0.05），细胞凋亡率、LC-3Ⅱ/LC-3Ⅰ比值和Beclin-1蛋白表达水平降低（P<0.05）。结论：柴胡疏肝散具有显著的抗抑郁作用，可能与拮抗大鼠海马神经元凋亡和降低自噬有关。

Influence of Bupleurum Liver-Coursing Powder on behavior,neuronal apoptosis and autophage in hippocampus of depressive rats

Objective To observe the effect of Bupleurum Liver-Coursing Powder on the neuronal apoptosis and autophage in hippocampus of depression model rats,and to explore the mechanism of its anti-depression effect.Methods 60male SD rats were randomly divided into control group,model group and drug intervention group(n=20).The depression model was produced by giving the rats chronic unpredicted mild stress.The rats in model group and control group received the same volume of normal saline,and the rats in drug intervention group received Bupleurum Liver-Coursing Powder solution on the basis of model.The depressional behavior was examined using sucrose preference test,tail-suspension test and Morris water maze.Flow cytometry was employed for the detection and quantification of the apoptotic cells in the hippcampus.The expressions of LC-3and Beclin 1were detected using Western blotting method.Results The pencertages of sucrose preference of the rats in model group and drug intervention group were significantly lower,while the tail-suspension immobility time and the excape latency time were higher than those in control group(P<0.05);the apoptotic rates of cells,the ratios of LC3-Ⅱ/LC3-Ⅰand the expression levels of Beclin-1were higher than those in control group(P<0.05).Compared with model group,the percentage of sucrose preference of the rats in drug intervention group was increased(P<0.05),the tail-suspension immobility time and the excape latency time were decreased(P<0.05),and the apoptotic rate of cells,the ratio of LC3-Ⅱ/LC3-Ⅰand the expression level were also decreased(P<0.05).Conclusion Bupleurum Liver-Coursing Powder has significant anti-depression effect,which may be related to inhibiting the apoptosis and degrading the autophage of neuros.