大肠癌组织中PARG与血管生成相关因子的关系

目的：初步探讨大肠癌组织中聚（腺苷二磷酸核糖）水解酶（PARG）与血管内皮生长因子（VEGF）和碱性成纤维细胞生长因子（bFGF）的关系。方法：采用免疫组织化学SP法检测43例大肠癌患者癌组织和10例对照切缘肠黏膜组织中聚(腺苷二磷酸核糖)聚合酶(PARP)、VEGF及bFGF。采用Western blotting检测PARG抑制剂单宁酸(GLTN)处理前后人结肠癌Lovo细胞PARG、PARP、NF-κB、VEGF及bFGF表达。结果：PARP、VEGF和bFGF在大肠癌组织中表达阳性率分别为97.67%(42/43)、79.07%(34/43) 和81.40%(35/43)，均高于对照组(P<0.05)。PARP分别与VEGF (r=0.396 8,P<0.05)和bFGF (r=0.561 0,P<0.05)表达呈正相关关系。在Lovo细胞中， GLTN处理组PARG、PARP、NF-κB、VEGF和bFGF的表达均低于GLTN未处理组(P<0.01)。结论：PARG可影响大肠癌血管生成相关因子表达，可能与其调节PARP进而影响NF-κB活性有关。

Relationship between PARG and angiogenesis related factors in colorectal carcinoma tissues

Objective To investigate the relationship between poly(ADP-ribose)glycohydrolase(PARG) and vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in colorectal carcinoma(CRC) tissues.Methods Immunohistochemical analysis was used to detect poly(ADP-ribose) polymerase(PARP),VEGF and bFGF in 43 cases of human colorectal carcinoma and 10 cases of intestinal mucosa of incisal margin(control group).Western blotting was used to detect the expressions of PARG,PARP,NF-κB,VEGF and bFGF in human Lovo cell line before and after the treatment of PARG inhibitor gallotannin (GLTN).Results The positive rates of PARP,VEGF and bFGF in colorectal carcinoma tissues were 97.67%(42/43),79.07%(34/43),and 81.40%(35/43),respectively;they were significantly higher than those in control group(P<0.05).The expression of PARP had positive correlation with VEGF (r=0.3968,P<0.05) and bFGF (r=0.5610,P<0.05).In GLTN-treated Lovo cell line,the expressions of PARG,PARP,NF-κB,VEGF and bFGF were markedly reduced compared with untreated cells (P<0.01).Conclusion PARG may affect the expression of the factors related to neovascularization in colorectal carcinoma.Its mechanism may be related to the regulation of PARP and affecting the NF-κB activity.