P21和survivin蛋白在脑胶质瘤组织中的表达及其临床意义

目的:探讨不同级别人脑胶质瘤组织和正常脑组织中P21和survivin蛋白的表达情况,分析其临床意义。方法:按2007年WHO制定的病理分级标准,将41份脑胶质瘤组织标本分为Ⅰ级9份、Ⅱ级14份、Ⅲ级10份和Ⅳ级8份,另外12份正常脑组织作为对照组,采用免疫组织化学法检测脑胶质瘤和正常脑组织中P21和survivin蛋白的表达情况,并分析两者表达的关系。结果:在正常脑组织和脑胶质瘤组织中P21及survivin蛋白阳性表达率分别为91.7%和53.7%及0%和51.2%,不同组织中P21和survivin蛋白的阳性表达率比较差异均有统计学意义(P<0.05)。Ⅰ-Ⅱ和Ⅲ-Ⅳ级脑胶质瘤组织中P21蛋白阳性表达率分别为69.6％及33.3%,而survivin蛋白阳性表达率分别为34.8%和72.2%,Ⅲ-Ⅳ级脑胶质瘤组织中P21蛋白阳性表达率明显低于Ⅰ-Ⅱ级,而survivin蛋白的阳性表达率明显高于Ⅰ-Ⅱ级(P<0.05)。结论:P21蛋白的表达缺失和survivin蛋白的过度表达与脑胶质瘤恶性程度有关联,P21蛋白有抑制脑胶质瘤发生发展的作用,而survivin蛋白在脑胶质瘤的恶变过程中起促进作用。

Expressions of P21 and survivin proteins in glioma tissue and their clinical significances

Objective To investigate the expressions of P21 and survivin proteins in human glioma tissue, and normal brain tissue, and to analyze their clinical significances. Methods According to the pathological standards formulated by the WHO in 2007,41 cases of glioma tissue samples were divided into 9 cases of grade Ⅰ,14 cases of gradeⅡ,10 cases of grade Ⅲ and 8 cases of grade Ⅳ;the other 12 cases of normal brain tissue were used as control group.The expressions of P21 and survivin proteins in human glioma tissue and normal brain tissue were examined with immunohistochemical method,and the relationship between them was analyzed. Results The positive expression rates of P21 and survivin proteins in normal brain tissue and glioma tissue were 91.7% and 53.7%,0% and 51.2%,and the differences between different tissues were statistically significant (P<0.05).The positive expression rates of P21 protein in the grade Ⅰ-Ⅱ and grade Ⅱ-Ⅲ glioma tissue were 69.6% and 33.3%,respectively,while the positive expression rates of survivin protein were 34.8% and 72.2%.The positive expression rate of P21 in the grade Ⅲ-Ⅳ glioma tissue was significantly lower than that in the grade Ⅰ-Ⅱglioma tissue, while the positive expression rate of survivin protein in the grade Ⅲ-Ⅳ glioma tissue was significantly higher than that in the grade Ⅰ-Ⅱ glioma tissue(P<0.05). Conclusion The loss of expression of P21 protein and the overexpression of survivin protein are associated with the malignant degree of gliomas.The P21 protein can inhibit the occurrence and development of glioma and the survivin protein can promote the development of glioma.