五味子乙素对染铅小鼠驱铅作用及其对驱铅过程中钙、铁和锌水平的影响

目的:探讨五味子乙素对染铅小鼠的驱铅作用及其对驱铅过程中钙、铁和锌水平的影响。方法:雄性ICR小鼠40只随机分为对照组(生理盐水灌胃,2%吐温溶液腹腔注射)、铅染毒组(Pb组,500 mg·kg^-1醋酸铅灌胃,2%吐温溶液腹腔注射)、五味子乙素组(SB组,生理盐水灌胃,4 mg·kg^-1五味子乙素混悬液腹腔注射)和五味子乙素驱铅组(SBPb组,500 mg·kg^-1醋酸铅灌胃,4 mg·kg^-1五味子乙素混悬液腹腔注射),每组10只。干预23 d后处死小鼠取材,计算小鼠体质量,原子吸收分光光度法检测各组小鼠血中铅、钙、铁和锌水平。结果:与对照组、SB和Pb组比较,SBPb组小鼠体质量明显降低(P<0.05);与染毒前比较,对照组、SB和Pb组小鼠染毒后体质量升高(P<0.05)。与对照组比较,Pb组小鼠睾丸脏器系数和血中铅水平升高(P<0.05);与对照组比较,Pb组小鼠血中锌水平降低(P<0.05);与SB组比较,Pb组小鼠血中锌水平降低(P<0.05);与Pb组比较,SBPb组小鼠血中钙和锌水平升高(P<0.05)。结论:五味子乙素能有效驱除染铅小鼠体内的铅,同时能抑制驱铅过程中导致的钙、铁和锌丢失。

Effect of schizandrin B on lead excretion in lead exposure mice and influence in levels of calcium,iron, and zinc in process of lead excretion

Objective To explore the effect of schizandrin B on lead excretion and its influence in the levels of calcium,iron,and zinc in the process of lead excretion. Methods 40 male ICR mice were randomly divided into control group (given saline and received 2%Tween by intraperitoneal injection),lead exposure group (Pb group,given 500 mg·kg^-1 lead acetate and received 2%Tween by intraperitoneal injection),schizandrin B group (SB group,given saline and received 4 mg·kg^-1 schizandrin B by intraperitoneal injection), and schizandrin B with lead group (SBPb group,given 500 mg·kg^-1 lead acetate and received 4 mg·kg^-1 schizandrin B by intraperitoneal injection),and there were 10 mice in each group.After treated for 23 d,the mice were sacrificed to measure the body weight,and the levels of lead,calcium,iron,and zinc in blood of the mice were detected by atomic absorption spectrometric method.The organ coefficients were calculated. Results Compared with control,SB and Pb group,the body weight of mice in SBPb group was decreased(P<0.05);compared with pre-treatment,the body weight of mice after treatment in control,SB,and Pb groups were increased(P<0.05).Compared with control group,the testes index and the level of lead in blood of the mice in Pb group were increased (P<0.05).Compared with control group,the level of zinc in blood of the mice in Pb group were decreased (P<0.05).Compared with SB group,the level of zinc in blood of the mice in Pb group were decreased (P<0.05).Compared with Pb group,the levels of calcium and zinc in blood of the mice in SB Pb group were increased (P<0.05). Conclusion Schizandrin B can excrete the lead in lead exposure mice and inhibit the loss of calcium,iron and zinc in the process of lead excretion.