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| 人骨形态发生蛋白-2腺病毒表达载体转染人骨髓基质干细胞及对其增殖的影响 | |
| 引用本文： | 李建军,刘建国,韩东,孙洪斌,卜丽莎,杨绍娟,张文岚,徐莘香.人骨形态发生蛋白-2腺病毒表达载体转染人骨髓基质干细胞及对其增殖的影响[J].吉林大学学报(医学版),2003,29(4):421-424. |
| 作者姓名： | 李建军 刘建国 韩东 孙洪斌 卜丽莎 杨绍娟 张文岚 徐莘香 |
| 作者单位： | 1. 吉林大学第一医院骨科,吉林,长春,130021 2. 吉林大学中日联谊医院手外科 3. 吉林大学中日联谊医院中心研究室 |
| 基金项目： | 国家自然科学基金;39800151; |
| 摘 要： | 目的 :用人骨形态发生蛋白 - 2腺病毒表达载体 ( Ad- BMP- 2 )转染人骨髓基质干细胞( h BMSC) ,以探讨基因转染对 h BMSC增殖的影响。方法 :将 Ad- BMP- 2转染体外培养的成人骨髓基质干细胞 ,利用免疫组化、原位杂交染色方法检测细胞内骨形态发生蛋白 - 2 ( BMP- 2 )的表达 ,蛋白印迹法检测转染后细胞培养液中 BMP- 2分泌蛋白表达。然后通过流式细胞仪分析其对细胞增殖的影响。结果 :转染后 ,h BMP- 2基因在 m RNA水平和蛋白水平均有表达。蛋白印迹法检测到培养液中有 BMP- 2蛋白阳性表达。转染 h BMP- 2基因后 ,细胞经流式细胞仪分析 ,S期细胞比例增多 ,说明细胞 DNA的合成增加。结论 :Ad- BMP- 2可高效转染 h BMSC,且促进细胞增殖 |
| 关 键 词： | 腺病毒 人 骨形态发生蛋白 骨髓基质干细胞 基因转染 |
| 文章编号： | 1671-587X(2003)04-0421-04 |
| 修稿时间： | 2002年9月12日 |
Effects of Transfection with an Adenoviral Vector Carrying the Human BMP-2 Gene on Human Bone Marrow Stromal Cells Proliferation in vitro |
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| LI Jian jun ,LIU Jian guo ,HAN Dong ,SUN Hong bin ,BU Li sha ,YANG Shao juan ,ZHANG Wen lan ,XU Xin xiang.Effects of Transfection with an Adenoviral Vector Carrying the Human BMP-2 Gene on Human Bone Marrow Stromal Cells Proliferation in vitro[J].Journal of Jilin University: Med Ed,2003,29(4):421-424. | |
| Authors: | LI Jian jun LIU Jian guo HAN Dong SUN Hong bin BU Li sha YANG Shao juan ZHANG Wen lan XU Xin xiang |
| Institution: | LI Jian jun 1,LIU Jian guo 1,HAN Dong 2,SUN Hong bin 2,BU Li sha 3,YANG Shao juan 3,ZHANG Wen lan 3,XU Xin xiang 1* |
| Abstract: | Objective:The Adenoviral vector carrying hBMP 2 was transfected into human bone marrow stromal cells (hBMSCs) in vitro to study the effects of the transfection on these cells proliferation. Methods:The expression of hBMP 2 in these cells after infection was determined by in situ hybridization and immunohistochemical analysis. BMP 2 productions were confirmed by western blot analysis of the supernatant collected from the cells. The changes in the cells proliferation were observed by flowcytometry. Results:The expression of hBMP 2 was confirmed in levels of mRNA and protein. The presence of BMP 2 was detected in the supernatant of infected cells. Cellular proportion in S period increased after infection, which indicates the increase of synthesis and propagation of cell DNA. Conclusion: Ad BMP 2 could infect hBMSC with high transduction efficiency and promote cells proliferation. |
| Keywords: | Adenoviruses human Bone morphogenetic protein Bone marrow stromal cells Gene transfection |
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