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二七方对急性放射性肠损伤大鼠肠道的保护作用及机制
引用本文:夏欣欣,李跃军,周冬枝,王瑞,韩萍萍. 二七方对急性放射性肠损伤大鼠肠道的保护作用及机制[J]. 广州中医药大学学报, 2017, 34(4). DOI: 10.13359/j.cnki.gzxbtcm.2017.04.021
作者姓名:夏欣欣  李跃军  周冬枝  王瑞  韩萍萍
作者单位:1. 西安交通大学第一附属医院,陕西西安,710061;2. 湖南中医药高等专科学校第一附属医院,湖南株洲,412000
基金项目:陕西省中医药管理局科研基金资助项目
摘    要:【目的】探讨二七方(由朱砂七、蜈蚣七、秦皮、黄柏、大黄等组成)对急性放射性肠损伤大鼠肠道的保护作用及机制。【方法】将60只健康清洁级成年SD大鼠随机分为正常组、模型组、白头翁汤组和二七方组,每组15只。采用6 MV高能X线10 Gy全腹部照射,复制急性放射性肠炎大鼠模型。二七方组、白头翁汤组分别给予相应药物二七方(剂量为8.85 g·kg~(-1)·d~(-1))、白头翁汤(剂量为4.69 g·kg~(-1)·d~(-1))灌胃治疗,正常组、模型组给予等体积生理盐水灌胃,连续7 d。造模结束后,取回盲部近端回肠,光镜下观察回肠组织病理学,采用图像分析仪测定绒毛高度、隐窝深度、黏膜和全层厚度,采用分光光度计测定回肠组织中MPO含量,采用免疫组化法测定回肠组织中caspase-3和PCNA的表达水平。【结果】二七方组和白头翁汤组回肠结构损伤较轻,绒毛高度、隐窝深度、黏膜厚度和全层厚度均显著大于模型组(P0.05),但2个给药组差异无统计学意义(P0.05);二七方组和白头翁汤组MPO含量较模型组显著降低(P0.05),且二七方组MPO含量显著低于白头翁汤组(P0.05);二七方组与白头翁汤组caspase-3、PCNA表达水平较模型组显著升高(P﹤0.05),但2组差异无统计学意义(P0.05)。【结论】二七方对急性放射性肠炎大鼠肠黏膜及组织损伤具有修复作用,其机理可能通过减轻局部炎症反应,促进肠黏膜上皮增殖,抑制上皮细胞凋亡而实现。

关 键 词:二七方/药理学  朱砂七  蜈蚣七  秦皮  急性放射性肠损伤/中药疗法  回肠/病理学  疾病模型,动物  大鼠

Protective Effect of Erqi Decoction on Intestinal Tract of Acute Radiation Intestinal Injury Rats and Its Mechanism
XIA Xin-Xin,LI Yue-Jun,ZHOU Dong-Zhi,WANG Rui,HAN Ping-Ping. Protective Effect of Erqi Decoction on Intestinal Tract of Acute Radiation Intestinal Injury Rats and Its Mechanism[J]. Journal of Guangzhou University of Traditional Chinese Medicine, 2017, 34(4). DOI: 10.13359/j.cnki.gzxbtcm.2017.04.021
Authors:XIA Xin-Xin  LI Yue-Jun  ZHOU Dong-Zhi  WANG Rui  HAN Ping-Ping
Abstract:Objective To investigate the protective effects of Erqi Decoction(EQD; mainly composed of Radix Aristolochiae Kaempferi, Radix Rhizoma Seu Flos Cypripedii, Cortex Fraxini, Cortex Phellodendri, Radix et Rhizoma Rhei) on the intestinal tract in rats with acute radiation intestinal injury and its mechanism. Methods Sixty SD rats were randomly divided into normal group, model group, EQD group and Baitouweng Decoction group (BD group), 15 rats in each group. The acute radiation enteritis model was established by exposing the whole abdomen to a total dose of 10 Gy of 6 MV higher-energy X-rays. EQD group and BD group were given intragastrical administration with corresponding medicine of EQD at the dose of 8.85 g·kg-1·d-1, BD at the dose of 4.69 g·kg-1·d-1 respectively, and the normal group and the model group were given intragastrical administration with the same volume of normal saline. The treatment lasted for 7 continuous days. After modeling, the morphological change of the proximal ileum tissue was observed under light microscope. Villus height, crypt depth, and thickness of the ileal mucosa and entire wall were measured by image analysis system. The myeloperoxidase (MPO) content in ileum tissue was determined by spectrophotometer, and the expression levels of caspase -3 and proliferating cell nuclear antigen (PCNA) in ileum tissue were determined by immunohistochemistry. Results EQD group and BD group had milder injuries of the ileal structure, and had higher villus height, crypt depth, and thickness of mucosa and entire wall than those in the model group (P <0.05), but there were no differences between the two medication groups(P > 0.05). MPO content in EQD group and BD group was decreased(P<0.05 compared with that in the model group), and MPO content in EQD group was lower than that in BD group. The expression levels of caspase-3 and PCNA were increased in EQD group and BD group(P < 0.05 compared with those in the model group), but there were no statistical differences between the two medication groups (P>0.05). Conclusion EQD has certain protective effects against radiation-induced intestinal damage, which mechanism is probably associated with relieving the local intestinal inflammatory reaction, accelerating intestinal epithelial cell proliferation, and inhibiting intestinal epithelial cell apoptosis.
Keywords:Erqi Decoction/pharmacology  Radix Aristolochiae Kaempferi  Radix Rhizoma Seu Flos Cypripedii  Cortex Fraxini  acute r adiation intestinal injury/TCD therapy  ileum/pathology  disease models  animal  rats
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