Genistein对体外培养兔软骨细胞一氧化氮表达的影响

目的：观察受体酪氨酸激酶抑制剂genistein对体外培养兔软骨细胞一氧化氮（NO）表达的影响。方法：兔膝关节软骨细胞传代培养,应用不同浓度genistein干预1、2、3、4天后,通过亚硝酸盐测定检测培养上清液NO表达水平。结果：形态学、甲苯胺蓝染色显示3代以内体外培养软骨细胞保持细胞表型的稳定。在genistein干预后上清液中NO水平下降,下降水平与genistein干预浓度和时间相关。结论：体外培养兔软骨细胞三代以内的细胞具有较好的活性,genistein能有效降低兔软骨细胞NO的表达水平。

Effects of Genistein on the Expression of Natric Oxide in Chondrocytes of Rabbits in Vitro

Objective：To investigate the effects of receptor tyrosine kinase inhibitor genistein on the expression of nitric oxide（NO） in chondrocytes of rabbits in vitro.Methods： The passage culture of rabbit articular cartilage cells was carried out with different concentrations of genistein and the cells cultured were divided into 7 groups：control,LPS,and LPS plus to different concentrations genistein（5,10,15,20 and 25μg/mL）.The expression of NO was detected in 1,2,3,4 days after intervention.Results： Morphology observation and toluidine blue staining results showed that a stable phenotype within 3 generations of the cartilage cells cultured.The expression of NO was decreased after the intervention of genistein in a concentration and time-dependent manner.Conclusion： Chondrocytes of rabbits could maintain its viability after 3 generation culture in vitro.Genistein can effectively inhibit the expression of NO.