回药爱康方协同化疗对小鼠Lewis肺癌细胞超微结构的影响

目的观察回药爱康方协同化疗对小鼠Lewis肺癌细胞超微结构的影响。方法把55只C57小鼠接种Lewis肺癌细胞24h后随机分为模型组、化疗组、爱康方组、爱康方低剂量加化疗组、爱康方高剂量加化疗组。治疗14d后,观察小鼠一般情况,完整剥离肿瘤组织称瘤重,电镜取材、制作,观察各组肿瘤细胞结构的改变。结果与模型组比较,其余各组小鼠一般情况较好,高剂量加化疗组最佳,小鼠瘤重较模型组轻,差异均有统计学意义(P<0.01);与模型组比较,其余各组的肺癌细胞形态都有不同程度的损伤,高剂量加化疗组细胞破坏最明显,出现大量细胞器坏死,染色质高度聚集。结论回药爱康方高剂量协同化疗可有效损伤肿瘤细胞。

Effective of Hui Medicine AikangFang Synergism Chemotherapy to Lewis Lung Carcinoma Cell Ultrastructure

Objective Observed effective of Hui medicine AikangFang synergism chemotherapy to mice Lewis lung cancer cell changes by electron microscope.Methods We uesed to 55 C57 mice which were inoculated Lewis lung carcinoma cells after 24h were randomly divided into model group,chemotherapy group,Hui medicine AikangFang;AikangFang low-dose and high-dose chemotherapy group.After 14d treatment,we observed the general situation of mice,completely stripping tumor tissue that the tumor,lung cells dissection,electron microscope drawn,fabrication,observed in each group changes in the structure of tumor cells.Results Compared with model group,the rest of group mice general situation were good,high-dose plus chemotherapy group was best,the mice tumor weight compared with model group were statistically significant(P < 0.01).Treatment groups of lung cancer cells have different degrees of morpho logical damage,high-dose chemotherapy group the most obvious cell damage,a large number of organelles necrosis,chromatin high degree of concentration.Conclusion Hui medicine AikangFang affects cancer cells,the high-dose chemotherapy can be effectively coordinated damage tumor cells.