水蛭、斑蝥对鸡胚绒毛尿囊膜(CAM)血管生成的影响

目的研究中药水蛭、斑蝥对鸡胚绒毛尿囊膜(CAM)血管生成的影响。方法将12只Wistar大鼠随机分为水蛭组(高低剂量组)、斑蝥组(高低剂量组)、血清组和空白组,中药水蛭、斑蝥各制成水煎液,灌胃7d,采血清备用。建立6d鸡胚绒毛尿囊膜血管模型,观察各组血清对鸡胚绒毛尿囊膜(CAM)血管生成的影响。结果水蛭高剂量组有明显地抑制新生血管数量的作用,与空白组比较,差异有统计学意义(P0.05);斑蝥高、低剂量组与空白组比较,亦能较强地抑制新生血管数(P0.05);水蛭、斑蝥高低剂量组有抑制血管生成面积的趋势,但差异无统计学意义(P0.05)。结论虫类中药水蛭、斑蝥对CAM血管生成有一定的抑制作用。

Impacts of Leech Hirudo and Blister Beetle Mylabris on the Angiogenesis of Chick Chorioallantoic Membrane

Objective：To study the impacts of leech hirudo and blister beetle mylabris on the angiogenesis of chick chorioallantoic membrane（CAM）.Methods：12 Wistar rats were randomized into leech hirudo group（high-dose and low-dose subgroups）,blister beetle mylabris group（high-dose and low-dose subgroups）,serum group and blank group.Leech hirudo and blister beetle mylabris were prepared as decoction separately and applied for gastric infusion for 7 days.Serum was collected in advance.6d CAM vessel model was established.The impacts of serums on the angiogenesis of CAM were observed in each group.Results：Leech hirudo high-dose group indicated the remarkable inhibition on the number of newly-generated vessel as compared with blank group,with statistical significant difference（P0.05）.Blister beetle mylabris high-dose and low-dose groups all inhibited strongly the number of newly-generated vessel as compared with blank group（P0.05）.The high-dose and low-dose groups of either leech hirudo or blister beetle mylabris indicated the inhibitory tendency on the area of angiogenesis,but without statistical significant difference in comparison（P0.05）.Conclusion：Leech hirudo and blister beetle mylabris in insect herbal medicine have a certain inhibition on the angiogenesis of CAM.