二黄汤方药半仿生提取法工艺条件的优选

目的用均匀设计优选二黄汤方药半仿生提取的工艺条件。方法采用均匀设计U9（91×33）表,在方药规格、煎提温度、煎提用水量、滤过、浓缩等条件相同的情况下,以3煎用水的pH值及总煎煮时间为考察因素,分子量≤1 000 Da提取物、黄芩苷、盐酸小檗碱、甘草次酸、总生物碱为指标,将各指标测得值进行标准化处理,并经加权求和后得到综合评判指标Y值,用JYSYSJ软件处理,优化该方药半仿生提取的工艺条件。结果经综合分析评判,优选出二黄汤方药SBE法较佳的工艺条件为：3煎用水的pH值依次为A=3.603 2、B=6.503 9、C=8.765 6,3煎总提取时间为D=4.947 3。结论结合生产实际,确定3煎用水的pH值依次为3.5、6.5、8.8,提取时间依次为2.0 h、1.5 h、1.5 h。验证性试验提取液的综合评价Y′值接近预测值,表明该方药用均匀设计优选出的SBE工艺参数可供在生产中试行。

Technique Optimization of Semi-Bionic Extraction for Erhuang Decoction

Objective To optimize the techniques of semi-bionic extraction（SBE）for erhuang decoction with uniform design.Methods The uniform design U9（91×33）table was used.In the same conditions,such as formula specification,decoction extraction temperature,water dose of decoction extraction,filtration and concentration,taking water pH values of three decoctions and the total decoction time as the investigation factors,and the extract of molecular weight ≤1 000 Da,baicalin,berberine hydrochloride,glycyrrhetinic acid and total alkaloids as the indicators,the values of all the indicators were standardized and Y value of comprehensive evaluation index was obtained after weighted summation.With JYSYSJ software management,the technique conditions of this formula SBE were optimized.Results With comprehensive analysis,the optimized techniques of SBE for erhuang decoction：pH values of three decoction A=3.603 2,B=6.503 9,C=8.765 6 successively;the total extraction time of three decoctions：D=4.947 3.Conclusion In association with the actual production,it is determined that pH values of three decoction are 3.5,6.5 and 8.8;and the extraction time is 2.0 h,1.5 h and 1.5 h successively.Y′ value of the comprehensive evaluation for the confirmatory test extract is near to the predicted value.All of those explain that SBE technique parameters optimized in the uniform design for this formula are applicable in production.