青蒿琥酯联合奥沙利铂对人结肠癌细胞HCT116的作用研究

目的观察青蒿琥酯(Artesunate,Art)与奥沙利铂(Oxaliplatin,L-OHP)联合用药对人结肠癌细胞株HCT116增殖和凋亡的影响。方法利用不同浓度单药Art、L-OHP及Art(6.25μg·mL-1)联合不同浓度L-OHP处理结肠癌HCT116细胞株48 h。采用四甲基偶氮唑盐(MTT)比色法分析细胞增殖变化;金正均Q值法判断两药联合效应,苏木精—伊红(HE)染色法观察细胞形态学变化;流式细胞仪检测细胞凋亡率。结果不同浓度Art和L-OHP对HCT116细胞株增殖均有抑制作用,且抑制率随药物浓度提高而增高(P0.05);联合用药表现出协同或相加效应;HE染色可见随Art浓度增加,细胞膜破裂,细胞核染色逐渐加深,细胞正常结构消失;Art(6.25μg·mL-1)与LOHP(0.5μg·mL-1)联合用药,联合组早期细胞凋亡率较单药Art(20.31±3.8)%、L-OHP(14.83±3.9)%显著提高(P0.05)。结论 Art与L-OHP联合用药能够显著抑制HCT116细胞株增殖并增强对细胞凋亡的诱导作用。

Study on HCT116 in Human Colon Cancer Cell Treated with Artesunate and Oxaliplatin

Objective ToobservetheimpactsofArtesunate（Art）andoxaliplatin（L-OHP）onthe proliferationandapoptosisofhumancoloncancerHCT116cells.Methods ThesimpleapplicationofArt and L-OHP of different concentrations,and the combined application of Art（6.25 μg·mL-1 ）and L-OHP of different concentrations were used separately for the treatment of HCT1 16 cells for 48h.MTT colori-metric method was used to analyze the proliferation of cells.Jin Zheng-jun′s Q value was adopted to judge the efficacy of the joint-medicinal application.HE staining method was used to observe the cell morphologi-calchanges.Flowcytometryinstrumentwasappliedtodetectcellapoptosis.Results ArtandL-OHPof different concentrations inhibited HCT 1 16 cells proliferation.The inhibition rate was improved with the con-centration increase（P〈0.05 ）.The joint-medicinal application presented synergistic inhibition or additive effect.HE staining indicated that with the concentration increase of Art,the cell membrane was ruptured,nu-clear staining was deepened gradually and the normal cellular structure disappeared.In the combination of Art （6.25 μg·mL-1 ）and L-OHP（0.5 μg·mL-1 ）,the early apoptosis rate was improved significantly as compared with single application of Art（20.31 ±3.8）%]or L-OHP（14.83 ±3.9）%]（〈0.05）. Conclusion ArtcombinedwithL-OHPcouldsignificantlyinhibitproliferationandinduceapoptosisof HCT1 16 cells.