CIK细胞与DC-CIK细胞抗肿瘤效应的对照研究

目的比较细胞因子诱导的杀伤细胞（cytokine-induced killer cells,CIK）与树突状细胞（dendritic cells,DC）结合CIK细胞抗肿瘤效应。方法外周血单个核细胞诱导DC和CIK细胞,将DC与CIK共培养,以CIK细胞单独培养为对照。用MTT法测定杀伤活性,流式细胞术分析免疫表型,ELISA法测定干扰素-γ（IFN-γ）、白介素-12（IL-12）、肿瘤坏死因子-α（TNF-α）的水平。结果 DC-CIK细胞增殖能力明显高于CIK细胞（P〈0.05）,DC-CIK细胞共培养后,CD3＋CD8＋、CD3＋CD56＋细胞比率较相同条件下CIK细胞组显著增多（P〈0.05）,共培养3 d,DC-CIK细胞上清液中IL-12、IFN-γ、TNF-α水平均比CIK细胞单独培养的水平高（P〈0.01）,DC-CIK细胞对白血病细胞与淋巴瘤细胞的杀伤率显著高于CIK细胞（P〈0.01）。结论 DC-CIK细胞比CIK细胞有更强的抗肿瘤效应。

A Comparative Study on Anti-Tumor Immune Response of CIK Cells and DC-CIK Cells

Objective To compare the anti-tumor immune response effect of cytokine-induced killer（CIK） cells and dendritic cell（DC）-CIK cells.Methods DC and CIK cells were induced from peripheral blood mononuclear cells.DC and CIK cells were co-cultured,while CIK cells were cultured alone as controls.The killing activity was detected by MTT assay;immunophenotype changes were analyzed by flow cytometry;the IL-12,IFN-γ and TNF-αlevels of the cultured supernatants were detected by ELISA kits.Results The proliferation capability of DC-CIK cells was significantly higher than that of CIK cells.Under the same condition,the ratio of double positive cells such as CD3＋CD8＋,CD3＋CD56＋ in CIK cells was significantly enhanced by co-cultured with DC cells.The levels of IL-12,IFN-γ and TNF-α in cultured supernatants of DC-CIK cells increased noticeably on day 3 as compared with CIK cells cultured alone.The activity of DC-CIK cells against leukemia cells were much higher than that of CIK cells and this effect showed a positive correlation with the effector-target ratio.Conclusion The activity of DC-CIK against anti-tumor cells is significantly higher than that of CIK cells.