血浆循环DNA在原发性干燥综合征和淋巴瘤中的比较分析

目的 比较血浆循环DNA（circulating DNA,cDNA）在原发性干燥综合征（primary Sj（o）gren＆#39;s syndrome,pSS）和淋巴瘤（Lymphomas）中的差异.方法 选用人类基因组管家基因GAPDH设计引物,测定pSS、淋巴瘤及正常对照组中血浆cDNA的值,同时测定血沉（ESR）、C-反应蛋白（CRP）、免疫球蛋白（IgA、IgG、IgM）,并评定pSS的活动指数（Sj（o）gren＆#39;s syndrome disease activity index,SSDAI）和损害指数（Sj（o）gren＆#39;s syndrome disease damage index,SSDDI）、淋巴瘤国际预后指标（Lymphoma international prognostic index,IPI）,分析cDNA与相关参数之间的关系.结果 3组间血浆cDNA两两比较：pSS组与淋巴瘤组、正常对照组比较差异均有统计学意义（P＜0.05）;pSS组中：cDNA浓度值与IgG呈正相关（r=0.333,P＜0.05）,cDNA浓度值与SSDAI呈正相关（r=0.551,P＜0.01）,cDNA浓度值与SSDDI无相关性（r=0.198,P＞0.05）,淋巴瘤组cDNA浓度值与IPI正相关（r=0.596,P＜0.01）;pSS组IgA、IgG均明显高于淋巴瘤组（P＜0.05）.结论 血浆cDNA在pSS及淋巴瘤中均升高,且两者之间有差异,作为pSS与淋巴瘤疾病鉴别的一种新的生物学标记,也可作为淋巴瘤预后及pSS疾病活动的观察指标,或许还可作为pSS向淋巴瘤发展的监测指标之一.

Investigation of plasma circulating DNA in patients with primary Sj（o）gren＆#39;s syndrome and in patients with lymphomas

Objective To explore the significance of peripheral blood plasma circulating DNA （cDNA） in groups of prima- ry Sj（igren＇ s syndrome（pSS） and Lymphomas. Methods The primer was designed by human genome housekeeper gene GAPDH. Levels of cDNA, erythrocyte sedimentation rate （ ESR）, C-reaction protein （CRP） and immunoglobulin （ IgA, IgG and IgM） in each group were detected. Meanwhile, the activity index and damage index of pSS, and Lymphoma interna- tional prognostic index （IPI） were assessed, and the relations between cDNA and disease activity were analyzed. Results The level of cDNA in pSS group （ 368.24 ± 196.68 ） ng/ml was significantly higher than that in Lymphoma group （20.46 ± 1.50） ng/ml, P 〈 0.05, and the cDNA level in Lymphoma group was significantly higher than that in con- trol group （24.56 ± 16.76 ）ng/ml, P 〈 0.05. In pSS group ： cDNA level was positively correlation with IgG （r = 0. 333, P 〈 0.05 = and Sjogren＇ s syndrome disease activity index（ SSDAI）, r = 0.551, P 〈 0.01. But there was no correlation between cDNA concentration and Sjogren＇ s syndrome disease damage index （ SSDDI）, r = 0. 198, P 〉 0.05. In Lymphomas group： cDNA level positively correlate with IPI（r =0. 596,P 〈0.01 ） ;the levels of IgA and IgG in pSS group were apparently higher than those in Lymphoma group （P 〈 0.05 ）. Conclusion The cDNA level may be a new biological marker for dis- tinguishing pSS from Lymphoma. The cDNA level can be considered as a prognostic indicator for lymphoma, moreover, it could be an observability index for pSS disease activity and the development of pSS for Lymphoma.