Kkay小鼠糖尿病肾病时ICAM-1的表达

目的:观察糖尿病肾病时(DN)细胞间黏附分子-1(ICAM-1)表达的变化。方法:22～24周龄:Kkay糖尿病鼠(KA)7只和Kkay非糖尿病鼠(KB)8只,测定血糖,以PAS染色观察各组:DN病变;对肾脏ICAM-1表达进行免疫组化染色和半定量图像分析;并以RT-PCR检测。肾脏ICAM-1 mRNA表达水平。结果:KA组出现明显糖尿病肾脏病变,其肾小球硬化指数(GI)240.00,明显高于KB对照组(118.36,P<0.05);免疫组化显示Kkay小鼠ICAM-1的表达与病理变化一致,其ICAM-1阳性着色面积(15.22％)高于对照组(4.38％,P<0.01),KA组ICAM-1 mRNA表达水平也高于KB组。结论:Kkay小鼠出现糖尿病肾病时伴有ICAM-1表达水平的增高,推测ICAM-1在糖尿病肾病发生发展中起一定作用。

The expression of intercellular adhesion molecule-1 in the diabetic nephropathy of Kkay mice

Objective:To study the expression of intercellular adhesion molecule-1 (ICAM-1) in the diabetic nephropathy of type 2 diabetes. Methods:Eight Kkay diabetic mice ( KA) and Kkay control mice ( KB) were treated with the same diets for four months and were sacrificed after the glucose were detected. The renal pathological changes were examined by periodic acid schiff (PAS) , and the glomerulosclerosis index (GI) was calculated. The expressions of ICAM-1 protein and mRNA were detected by immunohistochemical staining and RT-PCR. Results;The pathological changes of diabetic nephropathy in KB group were serious compared with KA group evidenced by morphological changes. It was showed that the diabetic nephropathy in kkay mice was companied by higher expression of ICAM-1 protein and mRNA levels in the glomeruli, and the mean positive area of ICAM-1 in KA group was higher than that in KB group (15. 22 vs4.38, P <0.01). Conclusions: The expression of ICAM-1 in diabetic nephropathy was enhanced, which may be related to the appearance and progression of nephropathy.