棕色脂肪剔除对高脂饲料诱导的肥胖小鼠体脂肪及血脂的影响

目的 观察棕色脂肪剔除对高脂饲料诱导的肥胖小鼠体脂肪及血脂的影响.方法 选择4-5周龄C57BL/6J雄性小鼠22只,随机分为肩胛下棕色脂肪剔除组和手术对照两组,每组11只,术后均喂饲高脂饲料16周.观察两组小鼠体质量、体脂肪、Lee氏指数、饲料消耗量、食物功效比及血脂的差异.结果 研究结束时,棕色脂肪剔除组小鼠的体质量、体脂肪量、Lee氏指数及血甘油三酯浓度均显著高于对照组(P＜0.05),棕色脂肪剔除组食物功效比显著低于手术对照组(P＜0.05),两组小鼠饲料消耗量及能量摄入量均无统计学差异(P＞0.05).结论 在棕色脂肪剔除情况下,高脂饲料喂养的小鼠体内脂肪蓄积增加、肥胖度增加、血甘油三酯浓度增加,提示小鼠棕色脂肪对防止肥胖的发生具有一定作用.

Effect of brown adipose tissue depletion on body fat and blood lipid induced by high fat diet in obesity mice

Objective To observe the effect of brown adipose tissue(BAT) depletion on body fat and blood lipid induced by high fat diet in obesity mice.Methods Twenty-two male C57BL/6J mice at the age of 4-5 weeks were randomly divided into BAT depletion group and control group,11 in each group.Their body fat mass,body fat,Lee’s index,diet consumption,food efficiency ratio and blood lipid were observed after they were fed with high fat diet for 16 weeks.Results At the end of study,the body fat mass,body fat,Lee’s index and triglyceride level were significantly higher while the food efficiency ratio was significantly lower in BAT depletion group than in control group(P<0.05),with no significant difference observed in diet consumption and energy intake between the two groups(P>0.05).Conclusion Fat is accumulated,obesity becomes severe and triglyceride level is higher in mice fed with high fat diet when their BAT is depleted,suggesting that BAT plays a certain role in preventing obesity.