中链脂肪酸对高脂饲料喂养的C57BL/6J小鼠高密度脂蛋白的影响

目的 观察中链脂肪酸(MCFA)对高脂饲料喂养的C57BL/6J小鼠血清、肝脏和小肠高密度脂蛋白(HDL)水平的影响.方法 36只高脂饲料喂饲小鼠,按照体质量随机分为2mg/kgMCFA组、4mg/kgMCFA组和4mg/kg长链脂肪酸(LCFA)组3组,灌胃给予以上脂肪酸2周后处死小鼠,称量体质量、体脂肪重和肝脏重,测定血清脂代谢指标,ELISA法检测血清、肝脏和小肠HDL、载脂蛋白A1(ApoA1)水平,实时定量RT-PCR法检测肝脏和小肠HDL及ApoAl mRNA表达水平.结果 低剂量MCFA组(2mg/kg)小鼠肝脏重显著低于LCFA组(4mg/kg),血清HDL-C/LDL-C比值、血清和肝脏HDL水平以及肝脏和小肠中HDL、ApoA1的mRNA表达均显著高于LCFA组(P＜0.05).高剂量MCFA组(4mg/kg)小鼠血清HDL-C和HDL水平、肝脏和小肠HDL mRNA表达显著高于LCFA组(P＜0.05).低剂量和高剂量MCFA组之间仅肝脏和小肠中的HDL、ApoA1mRNA表达有统计学差异(P＜0.05).结论 MCFA可升高高脂饲料喂养的C57BL/6J小鼠血清、肝脏和小肠HDL水平,上调其mRNA表达,对调节ApoAl的作用不明显.

Effect of medium-chain fatty acids on high-density-lipoprotein in C57BL/6J mice fed with high fat diet

Objective To observe the effect of medium-chain fatty acid(MCFA) on high-density-lipoprotein(HDL) in serum,liver and small intestine of C57BL/6J mice fed with high fat diet.Methods Thirty-six male C57BL/6J mice,fed with high fat diet,were randomly divided into 2mg/kg MCFA group,4mg/kg MCFA group and 4mg/kg long-chain fatty acid(LCFA) group,12 in each group.After the mice received intra-gastric fat acids(once a day for 2 weeks),they were killed.Their body,fat pad,and liver were weighed.Their HDL and apolipoproteinA1(ApoA1) levels in serum,liver,small intestine were measured by ELISA.Expression of HDL and ApoA1 mRNA in liver and small intestine was detected by real-time-PCR.Results The liver weight was significantly lower,while the serum HDL-C/LDL-C ratio and the expression level of HDL mRNA and ApoA1 mRNA in liver and small intestine were significantly higher in 2mg/kg MCFA group than in 4mg/kg LCFA group(P<0.05).The serum HDL-C and HDL levels,and the expression level of HDL mRNA in liver and small intestine were significantly higher in 4mg/kg MCFA group than in LCFA group(P<0.05).The expression of HDL mRNA and ApoA1 mRNA in liver and small intestine was significantly different between 2mg/kg and 4mg/kg MCFA groups(P<0.05).Conclusion MCFA can elevate the HDL level in serum,liver and small intestine,up-regulate the expression of HDL mRNA but not the expression of ApoA1 mRNA in liver and small intestine of C57BL/6J mice fed with high fat diet.