骨髓间充质干细胞移植促进糖尿病大鼠β细胞再生的机制

目的探索骨髓间充质干细胞（bone marrow mesenchymal stemcells，BMSCs）移植促进糖尿病大鼠模型中β细胞再生的机制。方法正常sD大鼠给予单次大剂量链脲佐菌素（streptozotocin，STZ）（65mg／kg）注射，3d后开始监测血糖，连续3d随机血糖〉28mmol／L者选为实验对象。所有实验对象随机分为对照组（n=15），BMSC移植组（n=15），BMSC培养上清移植组（n-15）以及BMSC示踪组（以Dil染料标记BMSCs，n=5），分别接受PBS、MSC、MSC培养上清及Dil—BMSC的移植。结果BMSC移植后30d左右血糖水平有明显改善（20．4±0．98）mmol／L，n=15]，胰岛素水平有明显提高（0．99±0．23）ng／ml，n=15]，p细胞质量明显增长。富含BMSC分泌因子的BMSC上清输注后，糖尿病大鼠血糖有一定程度的降低（26．2±1．53）mmol／L，n=15]，胰岛素水平有所恢复（0．341±0．08）ng／ml，n=15]，母细胞质量较对照组有所增加。在胰腺仅检测到小部分BMSC，这部分归巢于胰腺的BMSC中检测不到与胰岛素的共标。结论BMSC的分泌效应为BMSC促进糖尿病动物模型中β细胞再生的机制。

Mechanism of bone marrow mesenchymal stem cells transplantation in promoting β-cell regeneration in diabetic rats

Objective To study the mechanism of bone marrow mesenchymal stem cells （BMSC） transplantation in promoting β -cell regeneration in diabetic model of SD rats. Methods Three days after a single high streptozotocin （STZ） dose （65 mg/kg） was injected into normal SD rats, their blood glucose levels were measured. Those with their blood glucose level 〉 28 mmol/L for 3 consecutive days were randomly divided into control group （n=lS）, BMSC transplantation group （n=15）, BMSC supernatant transplantation group （n=lS）, and BMSC （marked with Dil stain） trace group （n=5）. Results The blood glucose level was significantly lower （20.4 ± 0.98 mmol/L, 26.2 ±1.53 mmol/L）, the serum insulin level was significantly higher （0.99 ± 0.23 ng/ml, 0.341 ± 0.08 ng/ml）, and the β cells regenerated faster in BMSC transplantation group and BMSC supernatant transplantation group than in control group 30 days after BMSC transplantation and after BMSC supernatant transplantation containing BMSC secreting factors. A small number of BMSC was detected but no co-marker of BMSC and insulin was detected in pancreas tissues of diabetic rats. Conclusion The secretion effect of BMSC is the mechanism of BMSC in promoting β -cell regeneration in diabetic rats.