转染SV40永生化基因的正常成人肝细胞药物转化功能的测定

目的了解转染SV40基因对正常成人肝细胞药物转化功能的影响。方法高效液相色谱法检测肝细胞对利多卡因的转化能力；Western blotting法检测两种肝细胞内细胞色素P450的表达。结果转染前、后肝细胞中利多卡因的浓度两者的利多卡因转化率无明显差异；两组肝细胞在蛋白电泳后都出现了54kU的蛋白条带，表明两种细胞均能表达细胞色素P450，均具有P4502E的免疫反应性。结论转染永生化基因前后的正常成人肝细胞均具有药物代谢所必需的细胞色素P450酶蛋白，仍保持了正常成人肝细胞的代谢功能。

Ability of SV40 gene transfection to transform normal adult liver cells

Objective To study the effect of SV40 gene transfection on ability of lidocaine to transform normal adult liver cells. Methods High-performance liquid chromatography was performed to detect the ability of lidocaine to transform liver cells. Western blotting was used to detect the expression of P450 in liver cells. Results No significant difference was found in the transformation rate of liver cells for lidocaine before and after SV40 gene transfection. Protein 54kU protein bands were observed in liver cells after electrophoresis, indicating that liver cells can express P450 and have immunoreactivity to P4502E. Conclusion Normal human liver cells before and after gene transfection have P450 protein that is necessary for drug metabolism and still maintain the metabolic function of normal adult liver cells.