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| 异丙酚对缺血再灌注损伤神经细胞的保护作用 | |
| 引用本文： | 陈娜,周建平,黄安宁,袁京,许秀丽,冯泽国.异丙酚对缺血再灌注损伤神经细胞的保护作用[J].军医进修学院学报,2011,32(12):1253-1256. |
| 作者姓名： | 陈娜 周建平 黄安宁 袁京 许秀丽 冯泽国 |
| 作者单位： | 1. 军事医学科学院毒物药物研究所,北京 100850;武警北京总队医院麻醉科,北京100027 2. 军事医学科学院毒物药物研究所,北京,100850 3. 武警北京总队医院麻醉科,北京,100027 4. 军事医学科学院毒物药物研究所,北京 100850;南开大学医学院,天津300071 5. 解放军总医院麻醉手术中心,北京,100853 |
| 基金项目： | “十一五”国家科技重大专项“重大新药创制”(2009ZX09103-722);第45批中国博士后科学基金特别资助~~ |
| 摘 要： | 目的 研究异丙酚对缺血再灌注损伤神经细胞的保护作用.方法 取12h新生Wistar大鼠海马细胞进行体外培养,建立原代海马细胞缺糖缺氧再给氧模型(oxygen glucose deprivation,OGD).观察海马细胞形态,苔盼蓝染色计算细胞存活率.建立SD大鼠脑缺血再灌注损伤模型,分为模型组、异丙酚用药组、正常对照组.观测各组动物术后神经症状评分、TTC染色测定脑梗死体积大小及HE染色.结果 海马细胞缺糖缺氧再给氧显示异丙酚用药组海马细胞存活数量较多,胞体较大突起保持稠密的神经网.异丙酚100μmol/L组细胞存活率为(56.2±3.7)％,较模型组(细胞存活率38.6％±4.3％)明显增高(P＜0.05).大脑中动脉阻断(middle cerebral artery occlusion,MCAO)模型24h神经症状评分,异丙酚用药组评分为(0.8±0.5)较模型组(2.3±0.5)显著降低(P＜0.05).TTC染色脑梗死体积异丙酚用药组为(123.21±11.04)mm3,较模型组(214.95±16.79)mm3显著降低(P＜0.01).HE染色可见异丙酚用药组正常细胞较多.结论 细胞和动物模型均显示异丙酚对缺血再灌注损伤后神经细胞具有保护作用. |
| 关 键 词： | 异丙酚 缺血再灌注损伤 海马神经元 |
Role of propofol in protection of neurons against ischemia/reperfusion injury |
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| CHEN Na , ZHOU Jian-ping , HUANG An-ning , YUAN Jing , XU Xiu-li , FENG Ze-Guo.Role of propofol in protection of neurons against ischemia/reperfusion injury[J].Academic Journal of Pla Postgraduate Medical School,2011,32(12):1253-1256. | |
| Authors: | CHEN Na ZHOU Jian-ping HUANG An-ning YUAN Jing XU Xiu-li FENG Ze-Guo |
| Institution: | 1Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences,Beijing 100850,China;2Department of Anesthesiology,Beijing Municipal Corps Hospital,Chinese People′s Armed Police Forces,Beijing 100027,China;3Medical College of Nankai University,Tianjin 300071,China;4Anesthesia and Operation Center,Chinese PLA General Hospital,Beijing 100853,China |
| Abstract: | Objective To study the role of propofol in protection of neurons against ischemia/reperfusion（I/R） injury.Methods Hippocampal cells of Wistar rats at the age of 12h were cultured in vitro and a hypoxia and reoxygenation model of primary hippocampal cells was established.Morphology of hippocampal cells was observed.Cell survival rate was calculated with trypan blue staining.An in vivo model of I/R injury was established.The rats were divided into middle cerebral artery occlusion（MCAO） model group,propofol treatment group,and normal control group.Longa′s neurological symptoms were scored.Cerebral infarction size was measured with TTC and H＆E staining.Results The survived hippocampal cells were characterized by a large number of cytodendrites with a dense neuron net in hypoxia and reoxygenation model group after propofol treatment.The cell survival rate was significantly higher in propofol（100μmol/L） treatment group than in MCAO model group（56.2±3.7 vs 38.6±4.3,P〈0.05）.The score of neurological symptoms was significantly higher in 24h MCAO model group than in propofol treatment group（0.8±0.5 vs 2.3±0.5,P〈0.05）.TTC and H＆E staining indicated that the infarction size was significantly smaller in propofol treatment group than in MCAO model group（123.21±11.04 vs 214.95±16.79,P〈0.05）.H＆E staining showed more normal cells in propofol treatment group than in MCAO model group.Conclusion Propofol can protect neurons against I/R injury. |
| Keywords: | propofol ischemia/reperfusion injury hippocampal neurons |
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