丙型肝炎患者外周血单个核细胞HCV和Fas抗原检测

目的：探讨丙型肝炎（丙型）患者外周血单个核细胞（ＰＢＭＣｓ）内丙型肝炎病毒抗原（ＨＣＶＡｇ）及Ｆａｓ抗原（ＦａｓＡｇ）表达状况及意义。方法：采用ＨＣＶ核心区及ＮＳ３区单克隆抗体，对２８例慢性丙肝患者ＰＢＭＣｓ进行免疫组化检测，并同步检测ＦａｓＡｇ。结果：ＨＣＶＡｇ及ＦａｓＡｇ阳性率分别为８５．７％（２４／２８）和８２．２（２３／２８），经相关分析显示。两者表达呈显著正相关（ｒ＝０．５４６，Ｐ〈０．

Detection of hepatitis C virus and Fas antigen in pheripheral blood mononuclear cells in patients with chronic hepatitis C

bjective:To study hepatitis C virus antigen (HCV Ag ) and Fas antigen (Fas Ag ) expression at the same time in pheripheral blood mononuclear cells (PBMCs) of patients with chronic hepatitis C. Methods:The anti HCV core and NS 3 monoclonal antibodies with Immuno histochmemistry technique was applied to detect HCV Ag and Fas Ag in the PBMCs of 28 patients. Results:Positive expression of HCV Ag and Fas Ag were 85 7% (24/28) and 82 2% (23/28) respectively. There was a obvious positive correlation between HCV Ag and Fas Ag ( r =0 546, P <0 01). The part of PBMCs with Fas Ag positive expression have changed in form of apoptosis. Conclusion:HCV could infect PBMCs and HCV could stimulate Fas Ag expression. HCV in PBMCs and apoptosis mediated through Fas Ag may lead to the persistent infection on patients.