一个新的胎肝发育相关因子cDNA的克隆与序列分析 |
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引用本文: | 钱建新.一个新的胎肝发育相关因子cDNA的克隆与序列分析[J].海南医学院学报,2001,7(4):195-196. |
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作者姓名: | 钱建新 |
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作者单位: | 海南医学院转基因实验室,海口,571101 |
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基金项目: | 由高等学校骨干教师资助(2000-65)海南百项农业新技术基金资助(1998-149) |
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摘 要: | 目的:从胎肝cDNA文库中,分离胎肝发育相关新cDNA。方法:分离纯化胎肝组织RNA,构建cDNA文库、T-A克隆、核酸自动测序及生物信息学分析。结果:分离克隆一个新的cDNA片段,编码71个氨基酸多肽,并且与肝脏生长/分化因子9部分同源。申请加入国际基因库,登录号为:GenbankAF157027。结论:该新cDNA(GenbankAF157027)编码的多肽可能调控肝脏的发育。
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关 键 词: | 基因 序列分析 分化 肝脏 |
修稿时间: | 2001年5月24日 |
THE CLONING AND SEQUENCE OF A NEW DEVELOPMENT-RELATED FACTOR IN HUMAN FETUS LIVER |
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Abstract: | Objective:To construct the cDNA library of fetus liver and isolate the new cD-NA clones.Method:RNA isolation,cDNA library construction,T-A cloning,DNA se quencing and bioinformatics analysis were conducted.Results:A new fragment of cDNA encoding a predicted pep-tide of71residues was isolated and cloned,which shared45%(16/35)amino acid and identified with the growth/differentiation factor9(GDF-9)of human liver.Conclusion:The peptide encoded by the new cDNA might be a growth/differenti ation factor of the liver. |
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Keywords: | Gene sequence analysis differentiation liver |
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