儿童肺炎支原体肺炎血清TNF-α、IL-6、IL-8和IL-10水平测定及临床意义

目的：探讨肺炎支原体肺炎（MPP）患儿血清肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）、白细胞介素-8（IL-8）和白细胞介素-10（IL-10）的浓度变化情况及其临床意义。方法：选择525例MPP患儿（研究组）和100例健康体检儿童（对照组）作为研究对象,研究组中69例存在胸腔积液,其中21例出现肺部纤维化改变,设为肺部有纤维化组,其余48例未出现肺部纤维化改变患者设为肺部无纤维化组。采用双抗体夹心酶联免疫吸附法（ELISA）检测并比较研究组治疗前后、对照组血清TNF-α、IL-6、IL-8和IL-10水平。结果：研究组治疗前血清TNF-α、IL-6、IL-8和IL-10水平均明显高于对照组,差异具有统计学意义（P〈0.05）;而研究组治疗后TNF-α、IL-6、IL-8和IL-10水平均较治疗前明显降低（P〈0.05）;肺部无纤维化组胸水TNF-α和IL-8水平高于其血清浓度,但差异无统计学意义（P〉0.05）;肺部有纤维化组胸水TNF-α和IL-8水平明显高于其血清浓度（P〈0.05）;而肺部有纤维化组胸水TNF-α和IL-8水平较无纤维化组明显增加（P〈0.05）。结论：TNF-α、IL-6、IL-8和IL-10与肺炎支原体感染具有一定相关性,且在肺部纤维化的形成过程中发挥重要的作用,可作为临床诊断、病情及预后判断依据,值得进行广泛的推广和应用。

Study on the clinical significance of Serum TNF-α, IL - 6, IL - 8 and IL - 10 Level in children with Mycoplasma pneumoniae pneumonia

Objective： To investigate the change of clinical significance of serum TNF-α, IL 6, IL - 8 and IL - 10 Level in Children with Mycoplasma pneumoniae, pneumonia. Methods： A total of 525 children with MPP were enrolled as research group and 100 health children were enrolled as control group. 69 cases of the research group had pleural effusion, of which 21 cases had pulmonary fibrosis and 48 cases without pulmonary fibrosis were set as pulmonary fibrosis group and non-pulmonary fibrosis group. Double antibody sandwich enzyme linked immunosorhent （ELISA） were applied to detect serum TNF-α, IL-6, IL-8 and 1L-10 levels for comparison. Results： Before treatment, serum TNF-α, IL-6, IL-8 and IL-10 levels of the research group were significant higher than that of control group （P〈0.05） ; after treatment, serum TNF-α, IL-6, IL-8 and IL-10 level were significantly lower than that before treatment （P〈0.05）. For patients without pulmonary fibrosis, TNF-α and IL-8 lev- els in pleural effusion were higher than that in serum, but there＇s no significant difference （P〉0. 05）. For those with pulmo- nary fibrosis, TNF-α and IL-8 levels in pleurai effusion were significantly higher than those without pulmonary fibrosis （P（ 0.05）. Conclusions： TNF-α, IL-6, IL-8 and IL-10 were correlated with Mycoplasma pneumoniae infection, and they play im- portant roles in the formation of pulmonary fibrosis. TNF-α, IL - 6, IL - 8 and IL - 10 can be used as indicators for clinical di- agnosis, prognosis and judgement of the disease.