盐酸格拉司琼体外抑菌作用的实验研究

目的:观察盐酸格拉司琼在体外对大肠埃希菌、金黄色葡萄球菌、铜绿假单胞菌抑菌作用的影响。方法:将盐酸格拉司琼注射液用无菌生理盐水稀释至终浓度分别为0.5、0.25、0.125和0.0 625mg/mL,与大肠埃希杆菌、金黄色葡萄球菌、和铜绿假单胞菌培养,每个实验重复三遍,所有菌株的稀释的系列测定均进行两次。结果:格拉司琼对大肠埃希菌、金黄色葡萄球菌、铜绿假单胞菌均有明显的抑菌作用,格拉司琼对大肠埃希菌的抑菌作用随着格拉司琼浓度的增加而增加,至0.0 625mg/mL时仍有明显的抑菌作用(P<0.05)。格拉司琼对金黄色葡萄球菌和铜绿假单胞菌的抑菌作用在格拉司琼浓度为0.125mg/mL时消失。结论:格拉司琼在体外可以抑制大肠埃希菌、金黄色葡萄球菌、铜绿假单胞菌的生长,对于预防麻醉相关的感染可能有一定的临床意义。

Effects of antibacterial activity of granisetron in vitro

Objective:To study the antibacterial activity of granisetron against Escherichia coli,Staphylococcus aureus and Pseudomonas aeruginosa in vitro.Methods:A series of solutions including 0.5 mg/mL,0.25 mg/mL,0.125 mg/mL and 0.0625 mg/mL of granisetron were prepared with normal saline,and then mixed with standard bacterial inocula of Escherichia coli,Staphylococcus aureus and Pseudomonas aeruginosa.Every test was repeated for three times.Results:Granisetron inhibites the growth of Escherichia coli,Staphylococcus aureus and Pseudomonas aeruginosa,and the inhibition activity increased with the concentration of Granisetron.The inhibition of Granisetron against the growth of Staphylococcus aureus and Pseudomonas aeruginosa disappeared at a concentration of 0.125 mg/mL(P<0.05).Conclusions:Granisetron can inhibit the growth of Escherichia coli,Staphylococcus aureus and Pseudomonas aeruginosa in vitro,thus it may be valuable in the prevention of anesthesia related infection.