C57BL/6J小鼠及鸡胚B16细胞移植瘤血管生成拟态的观察及其MMP7和MMP9的表达

目的]探讨黑色素瘤B16细胞鸡胚和小鼠移植瘤组织中MMP7和MMP9的表达与血管生成拟态形成的关系,初步探讨宿主微环境对肿瘤细胞的影响.方法]分别建立黑色素瘤B16细胞鸡胚和小鼠移植瘤模型,通过HE切片观察血管生成拟态的分布情况,利用免疫组化方法检测MMP7和MMP9的表达.结果]B16细胞鸡胚和小鼠移植瘤组织中均可见血管生成拟态,并且前者显著多于后者.鸡胚移植瘤组织和小鼠移植瘤组织中MMP7的阳性细胞百分比分别为34.55±8.98和15.27±4.21,前者显著高于后者(P＜0.01),MMP9的阳性细胞百分比分别为16.38±3.34和10.53±1.95,前者显著高于后者(P＜0.01).结论]鸡胚移植瘤可用于研究肿瘤生长早期的生物学行为,MMP7和MMP9的表达可能与肿瘤生长早期血管生成拟态的形成有关.

Vasculogenic mimicry observation on chick embryo and C57BL/6L transplanted B16 melanoma cells and the expression of MMP7 and MMP9 in two transplanted tumors

【Objective】 To investigate the expression of MMP7 and MMP9 in transplanted melanoma B16 cells in chick embryo and C57BL/6L mice and to investigate the relationship between their expression and vasculargenic mimicry and to investigate the effect of different host microenviroment on tumor cell.【Methods】 Transplanted melanoma B16 cells in chick embryo and C57BL/6L mice were established.Vesculargenic mimicry were observed by HE stained sections.Expression of MMP7 and MMP9 were detected by immunohistochemistry.【Results】 Vesculogenic mimcry could be observed in both chick embryo and transplanted melanoma B16 cells and those in chick embryo were significantly higher than those in C57BL/6J mice.The positive expression percentage of MMP7 in chick embryo and C57BL/6L mice were 34.55±8.98 and 15.27±4.21,respectively,the former was significantly higher than that of the latter（t = 7.38,P 0.01）.The positive expression percentage of MMP9 in chick embryo and C57BL/6L mice were 16.38±3.34 and 10.53±1.95,respectively,the former was significantly higher than that of the latter（t = 5.75,P 0.01）.【Conclusion】 Transplanted tumor of chick embryo can be used to study the biological behavior of early stage tumor and the high expression of MMP7 and MMP9 may conduce to the formation of vasculogenic mimicry.