电磁场干预大鼠骨髓间充质干细胞向心肌方向诱导分化的研究

【目的】观察脉冲电磁场（PEMFs）对5-氮胞苷（5-aza）诱导大鼠骨髓间充质干细胞（MSCs）向心肌样细胞分化的影响。【方法】50Hz脉冲低频电磁场刺激5-aza诱导7d后的MSCs，根据不同的感应强度和作用的时间分为A、B、C组和1、2、3、4亚组，设未暴露PEMFs的为对照组。通过Western印迹法检测不同条件下心肌肌钙蛋白T的变化。【结果】0．5、1和5mT组中20min、30min各亚组cTnT的表达量较对照组，5mT组在10min的表达量减少。【结论】50Hz的0．5～5mT感应强度的PEMFs作用20～30min为促进体外5-aza诱导MSCs向心肌方向的分化的有效窗口。

Study the effects of pulsed electromagnetic fields on the induction of rat marrow mesenchymal stem cells to differentiate into cardiomyocytes-like cells

Objective] To study the effects of pulsed electromagnetic fields on the differentiation of rat marrow mesenchymal stem cells into cardiomyocytes-like cells induced with 5-azacytidine. Methods] Rat marrow mesenchymal stem cells induced for 7 days with 5-azacytidine was exposed or unexposed to 50 Hz pulsed electromagnetic fields, and then randomly divided into A, B, C group and 1, 2, 3, 4 subgroup accreding to different inductance intensity and different expoure time, Unexposed under pulsed electromagnetic fields was used as control group. The protein expression of cardiac troponin T （cTnT） was determined by Western blotting. Results] The expressions of cardiac troponin T enhanced significantly under 0.5 mT, 1 mT and 5 mT inductance in- tensity for 20 - 30 min and reduced significantly under 5 mT inductance intensity for 10min. Conclusions] 0.5 mT and lint in- dactance intensity for 20 ~ 30 mind 50 Hz pulsed electromagnetic is the effective condition for promoting marrow mesenehymal stem cells differentiate into cardiomyocyte-like cells.