首页 | 本学科首页   官方微博 | 高级检索  
检索        

气相色谱内标法同时测定土木香中土木香内酯、异土木香内酯的含量
引用本文:王燕,王芸,孙文基.气相色谱内标法同时测定土木香中土木香内酯、异土木香内酯的含量[J].武警医学院学报,2010,19(7):527-529.
作者姓名:王燕  王芸  孙文基
作者单位:1. 西北大学陕西省生物医药重点实验室,陕西,西安,710069
2. 西安陆军学院门诊部,陕西,西安,710108
摘    要:目的]建立内标法测定土木香药材中土木香内酯、异土木香内酯的含量.方法]SE-30毛细管柱(30m×0.32mm×0.25μm);FID检测器温度为260℃;进样口温度240℃程序升温:初始温度50℃,以20℃/min的速率升温至170℃,保持28min,再以25℃/min的速率升温至250℃,保持1 min;以尼泊金丁酯为内标物定量.结果]土木香内酯与异土木香内酯分离良好,在0.6~4.2mg/ml范围内,呈良好的线性关系(r=0.9999,n=5).结论]本法简便、灵敏、专属性强、重复性好,可用于土木香药材的含量测定,控制其质量.

关 键 词:土木香  气相色谱法  内标法  土木香内酯  异土木香内酯

Gas chromatographic internal standard method for the determination of alantolactone and isoalantolactone in Radix Inulae Heleni
WANG Yan,WANG Yun,SUN Wen-ji.Gas chromatographic internal standard method for the determination of alantolactone and isoalantolactone in Radix Inulae Heleni[J].Acta Academiae Medicinae CPAPF,2010,19(7):527-529.
Authors:WANG Yan  WANG Yun  SUN Wen-ji
Institution:(Biomedicine Key Laboratory of Northwest University,Xi'an 710069, China)
Abstract:Objective] To establish a capillary GC method for the determination of alantoctone and isoalantoctone in Radix Inulae Heleni. Methed]The analytical column was SE-30 capillary column(30 m × 0.32 mm × 0.25 μm)with an FID detector. The injector temperature was 240 ℃. The detector temperature was set at 260 ℃. The column temperature was programmed from 50 ℃at a rate of 20 ℃/min to 170 ℃ (holding 28 min), then raised to 250 ℃ at 25 ℃/min. The final temperature was maintained for 1 min. Results] The linear ranges of alantoctone and isoalantoctone were 0.6 - 4.2 mg/ml (r = 0.9999), which showed good linearity. Conclutions] The methed is simple, specificity, accurate and reliable, and can be used for quality control of Tumuxiang.
Keywords:Radix Inulae Heleni  Tumuxiang  Gas chromatography  Alantolactone  Isoalantoctone
本文献已被 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号