Smad泛素化调节因子1与胃腺癌发生相关性研究

【目的】探讨Smad泛素化调节因子1（Smad ubiquitination regulatory factor 1,SMURF1）在胃腺癌中的表达水平,从而证明其在胃腺癌发生中的可能作用。【方法】以相应癌旁正常组织为对照,应用实时定量聚合酶链式反应方法和Western blotting印迹杂交方法检测101例胃腺癌组织中SMURF1 mRNA和蛋白表达水平,并应用统计学方法比较组间差异。【结果】肿瘤组SMURF1 mRNA表达水平均值为0.921±0.293,癌旁组织均值为0.611±0.251,在检测的101例标本中,肿瘤组织SMURF1mRNA水平高于相应癌旁组织的有71例,占70%;肿瘤组SMURF1蛋白表达均值为0.665±0.223,癌旁组织为0.416±0.203,肿瘤组织SMURF1蛋白表达高于相应癌旁组织的有78例,占77%。经统计分析,SMURF1 mRNA与蛋白表达水平肿瘤组与对照组组间差异均显著（P〈0.05）。【结论】胃腺癌中SMURF1表达水平升高,其可能在胃腺癌的发生中发挥一定作用。

Study on the correlation between Smad ubiquitination regulatory factor 1 and gastric adenocarcinoma

Objective]To assess the expression level of Smad ubiquitination regulatory factor 1 （SMURF1） in gastric adenocarcinoma, so as to probe its possible role in the development of gastric adenocarcinoma. Methods ] With the paired nomlal tissues as control, the expression of SMURF1 mRNA and protein was detected in 101 cases of gastric adenocarcinoma with real-time polymerase chain reaction （real-time PCR） and Western blotting assays. Statistic analysis was carried out to compare the differences between groups. Results] The average relative expression level of SMURF1 mRNA in tumor group was 0.921 ± 0.293, and that in paired normal tissues was 0.611 ± 0.251. In 71 cases, the expression of SMURF1 mRNA was higher in tumor tissues than in paired normal tissues, accounting for 70%. The average SMURFI protein expression level was 0.665 ± 0.223 in tumor group, and that in control group was 0.416 ± 0.203. In 78 cases, the expression of SMURF1 protein was higher in tumor tissues, accounting for 77%. The differences of both SMURFI mRNA and protein levels between tumor group and control group were statistically significant （P 〈 0.05）. Conclusion] The expression of SMURF1 was up-regulated in gastric adenocarcinoma, which may play a role in the development of gastric carcinoma.