大鼠中枢神经NOS阳性触液神经元的形态学观察

目的：探讨脑脊液中一氧化氮（NO）的来源及其分泌的形态学基础，了解中枢神经一氧化氮合酶（NOS）阳性触液神经元的分布特征。方法：将12只成年雄性Wistar大鼠脑冠状位切片行NADPH－组织化学方法染色，对环脑室系统及脑表面NOS阳性触液神经元作了观察。结果：NOS阳性触液神经元主要见于中脑水管和第三脑室（3V）。在中脑水管，触液神经纤维来自中脑的中央灰质，特别是其背外侧部；来自脑干的被盖痛外侧核和中缝背核。在3V，NOS阳性纤维主要来自室调周核、室旁核，NOS阳性胞体有位于室管膜下（远位）、膜内和膜上3种。阳性纤维密集地分布于室管膜内。在中央管、第四脑室底、脑表面也有少量触液神经元。结论：脑脊液中的NO来自室管周及脑表面NOS触液神经元之合成和分泌。

Morphological observation of NOS positive CSF-contacting neurons in central nervous system of rat

Objective:To investigate the resources of nitric oxide(NO)of the cerebrospinal fluid(CSF)and its morphology of neuroendocrine,as well as the distribution of nitric oxide synthase(NOS)positive CSF contacting neurons.Methods:The coronal slices of brains and brain stems of 12 Wistar rats were observed using the NADPH d histochemistry method.Results:The NOS positive CSF contacting neurons were mainly at the cerebral aqueduct and the third ventricle(3V).The cell body was located in the central gray substance(especially in the dorsolateral part),dorsal raphe nucleus,dorsolateral tegmental nucleus,and the periventricular nucleus,paraventricular nucleus and the supraoptic nucleus.The NOS positive fibers were extended into the ependyma of the cerebral aqueduct and thd 3V.The NOS containing neurons and the fibers were also seen about the central canal,fundus of fourth ventricle and the surface of the brain.Conclusion:The NO in the CSF comes from the NOS positive CSF contacting neurons surrounding the central canal,third and fourth ventricles,and of the surface of brain.These NOS positive neurons and their fibers synthesize and secrete the NO into the CSF.