首页 | 本学科首页   官方微博 | 高级检索  
检索        

京尼平苷对大鼠前列腺成纤维细胞增殖的影响
引用本文:晋玉章,马勇,王洪敏.京尼平苷对大鼠前列腺成纤维细胞增殖的影响[J].武警医学院学报,2010,19(3):175-177.
作者姓名:晋玉章  马勇  王洪敏
作者单位:1. 武警医学院机能实验中心,天津,300162
2. 武警医学院科研部,天津,300162
摘    要:【目的】研究京尼平苷对大鼠前列腺成纤维细胞增殖的影响,并探讨其机制。【方法】培养SD大鼠前列腺成纤维细胞,取对数生长期的细胞随机分成4组:对照组、京尼平苷10mg/L、20mg/L和40mg/L组。不同浓度的京尼平苷孵育大鼠前列腺成纤维细胞48h后,采用MTT法检测细胞增殖,流式细胞仪检测细胞凋亡,ELISA法测定Ⅰ型胶原、Ⅲ型胶原与转化生长因子β1(TGF-β1)蛋白的含量。【结果】与对照组比较,京尼平苷20mg/L和40mg/L可显著抑制大鼠前列腺成纤维细胞的增殖(P〈0.01),促进其凋亡(P〈0.01),同时减少TGF-β1的分泌和胶原的合成(P〈0.05或P〈0.01)。【结论】京尼平苷可抑制大鼠前列腺成纤维细胞的增殖,可能与其减少TGF-β1的分泌和胶原的合成、促进前列腺成纤维细胞的凋亡有关。

关 键 词:京尼平苷  成纤维细胞  胶原蛋白  转化生长因子β1

Effects of geniposide on proliferation of prostate fibroblast in rats
JIN Yu-zhang,MA Yong,WANG Hong-min.Effects of geniposide on proliferation of prostate fibroblast in rats[J].Acta Academiae Medicinae CPAPF,2010,19(3):175-177.
Authors:JIN Yu-zhang  MA Yong  WANG Hong-min
Institution:JIN Yu-zhang,MA Yong,WANG Hong-min(Experiment Center of Function,Medical College of Chinese People\'s Armed Police Force,Tianjin 300162,China)
Abstract:Objective] To investigate effects and mechanism of geniposide on proliferation in fibroblast of rats.Methods]The fibroblast of rats were isolated.The fibroblast which were in exponential phase of growth were divided randomly into 4 groups:control group,geniposide 10mg/L,20mg/L and 40mg/L groups.After incubated with different dosages of geniposide,the proliferation of fibroblast was detected by thiazole blue(MTT)colorimetric assay.Apoptosis was analyzed by flow cytometry.The content of collagen Ⅰ,Ⅲ and TGF-β1 were measured by the ELISA.Results] Compared with control group,the proliferation of prostate fibroblast in rats were inhibited significantly by geniposide 20mg/L and 40mg/L respectively(P0.01).Apoptosis was increased(P0.01,respectively)and the content of collagen Ⅰ,Ⅲ and TGF-β1 were decreased(P0.05 or P0.01,respectively)by geniposide 20mg/L and 40mg/L,respectively.Conclusions] Geniposide can inhibit proliferation of prostate fibroblast in rats.It may be associated with reduced secretion of TGF-β1 and collagen synthesis,promoting prostate fibroblasts apoptosis.
Keywords:Geniposide  Fibroblast  Collagen protein  Transforming growth factor β1
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号